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Element distribution and iron speciation in mature wheat grains (Triticum aestivum L.) using synchrotron X-ray fluorescence microscopy mapping and X-ray absorption near-edge structure (XANES) imaging

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dc.contributor.authorDe Brier, N.
dc.contributor.authorGomand, S.V.
dc.contributor.authorDonner, E.
dc.contributor.authorPaterson, D.
dc.contributor.authorSmolders, E.
dc.contributor.authorDelcour, J.A.
dc.contributor.authorLombi, E.
dc.date.issued2016
dc.descriptionData source: Supporting information, http://onlinelibrary.wiley.com/doi/10.1111/pce.12749/abstract#footer-support-info
dc.description.abstractSeveral studies have suggested that the majority of iron (Fe) and zinc (Zn) in wheat grains are associated with phytate, but a nuanced approach to unravel important tissue-level variation in element speciation within the grain is lacking. Here, we present spatially resolved Fe-speciation data obtained directly from different grain tissues using the newly developed synchrotron-based technique of X-ray absorption near-edge spectroscopy imaging, coupling this with high-definition mu-X-ray fluorescence microscopy to map the co-localization of essential elements. In the aleurone, phosphorus (P) is co-localized with Fe and Zn, and X-ray absorption near-edge structure imaging confirmed that Fe is chelated by phytate in this tissue layer. In the crease tissues, Zn is also positively related to P distribution, albeit less so than in the aleurone. Speciation analysis suggests that Fe is bound to nicotianamine rather than phytate in the nucellar projection, and that more complex Fe structures may also be present. In the embryo, high Zn concentrations are present in the root and shoot primordium, co-occurring with sulfur and presumably bound to thiol groups. Overall, Fe is mainly concentrated in the scutellum and co-localized with P. This high resolution imaging and speciation analysis reveals the complexity of the physiological processes responsible for element accumulation and bioaccessibility.
dc.identifier.citationPlant, Cell and Environment, 2016; 39(8):1835-1847
dc.identifier.doi10.1111/pce.12749
dc.identifier.issn0140-7791
dc.identifier.issn1365-3040
dc.identifier.urihttps://hdl.handle.net/11541.2/124369
dc.language.isoen
dc.publisherWiley-Blackwell Publishing Ltd.
dc.relation.funding‘Fonds voor Wetenschappelijk Onderzoek Vlaanderen’ (FWO, Brussels, Belgium) Travel grant
dc.rightsCopyright 2016 JohnWiley & Sons Ltd
dc.source.urihttps://doi.org/10.1111/pce.12749
dc.subjectaleurone
dc.subjectcrease region
dc.subjectembryo
dc.subjectnutrient elements
dc.titleElement distribution and iron speciation in mature wheat grains (Triticum aestivum L.) using synchrotron X-ray fluorescence microscopy mapping and X-ray absorption near-edge structure (XANES) imaging
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9916105192801831

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