Laboratory diagnosis of Mycoplasma pneumoniae infection: 1. Direct detection of antigen in respiratory exudates by enzyme immunoassay

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dc.contributor.authorKok, T.W.
dc.contributor.authorVarkanis, G.
dc.contributor.authorMarmion, B.P.
dc.contributor.authorMartin, J.
dc.contributor.authorEsterman, A.
dc.date.issued1988
dc.description.abstractDirect and indirect antigen capture enzyme immunoassays (Ag-EIA) have been developed for the detection of Mycoplasma pneumoniae in nasopharyngeal aspirates or sputum from respiratory infection. The sensitivity of the two Ag-EIA were similar, but the indirect method using polyclonal rabbit and guinea-pig antisera was more convenient. The Ag-EIA had a detection limit of 10(4-4.5) colony-forming units/ml of sample. It was specific for M. pneumoniae and gave a low level response with M. genitalium. There were no cross-reactions with 10 other species of mycoplasmas. Tests with a wide range of bacteria and chlamydia group antigen, representing agents sometimes found in the respiratory tract, were also negative. At the current level of development, the Ag-EIA detected about 90% of specimens that were also positive for culture; 43% of specimens from culture-negative--seropositive patients gave a positive result. The overall pattern of results indicated that while antigen detection is a quick and effective substitute for the slow culture method, serological examination for specific IgM antibody is also necessary to give a complete diagnostic coverage.
dc.description.statementofresponsibilityT.-W. Kok, G. Varkanis, B. P. Marmion, J. Martin and A. Esterman
dc.identifier.citationEpidemiology and Infection, 1988; 101(3):669-684
dc.identifier.doi10.1017/S0950268800029551
dc.identifier.issn0950-2688
dc.identifier.issn1469-4409
dc.identifier.orcidEsterman, A. [0000-0001-7324-9171]
dc.identifier.urihttp://hdl.handle.net/2440/114947
dc.language.isoen
dc.publisherCambridge University Press
dc.rights© Cambridge University Press 1988
dc.source.urihttps://doi.org/10.1017/s0950268800029551
dc.subjectNasopharynx
dc.subjectMycoplasma pneumoniae
dc.subjectAntigens, Bacterial
dc.titleLaboratory diagnosis of Mycoplasma pneumoniae infection: 1. Direct detection of antigen in respiratory exudates by enzyme immunoassay
dc.typeJournal article
pubs.publication-statusPublished

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