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An on-demand antibacterial hydrogel for precise and rapid elimination of bacterial infection in a murine partial thickness scald burn wound

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dc.contributor.authorHaidari, H.
dc.contributor.authorAmsalu, A.
dc.contributor.authorVasilev, K.
dc.contributor.authorCowin, A.J.
dc.contributor.authorKopecki, Z.
dc.date.issued2024
dc.descriptionData source: Supplementary materials, https://doi.org/10.1016/j.apmt.2024.102237
dc.description.abstractBurn wounds trigger prolonged inflammation, impair healing, and result in a high mortality rate. The effective management of burn patients requires a targeted approach to regulate the wound microenvironment, maintaining sterility while fostering conditions conducive to healing and functionality. Here, we developed a targeted antibacterial pH/temperature-responsive silver nanoparticle (AgNP) hydrogel triggering the release of silver ions based on changes in the burn wound microenvironment. The delivery system not only exerts a strong antibacterial effect owing to the localization and interfacial interaction of ultrasmall AgNPs against the bacterial surface and deeply embedded cells within the biofilm but also downregulates the bacterial pore-forming genes, thereby overwhelmingly deactivating bacterial responses through a multifaceted mechanism of action. We demonstrate that the application of AgNP hydrogel results in pH-dependent bacterial killing and elimination of over 95 % of pathogens while being non-toxic to mammalian cell viability and migration. Furthermore, the in vivo preclinical burn infection murine model demonstrates the eradication of S. aureus infection leading to a progressive burn repair supported by increased collagen deposition, anti-inflammatory properties, and increased burn angiogenesis. Overall, this strategy provides a targeted approach to addressing drug-resistant chronic burn infections, thereby contributing to improved management of burn injuries.
dc.identifier.citationApplied Materials Today, 2024; 38(102237):1-15
dc.identifier.doi10.1016/j.apmt.2024.102237
dc.identifier.issn2352-9407
dc.identifier.issn2352-9407
dc.identifier.orcidCowin, A.J. [0000-0003-2885-2080]
dc.identifier.orcidKopecki, Z. [0000-0002-8072-4222]
dc.identifier.urihttps://hdl.handle.net/11541.2/38947
dc.language.isoen
dc.publisherElsevier BV
dc.relation.fundingChannel 7 Children's Research Foundation Fellowship
dc.rightsCopyright 2024 The Author(s). This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/)
dc.source.urihttps://doi.org/10.1016/j.apmt.2024.102237
dc.subjectburn infection
dc.subjectlocalized delivery
dc.subjecton-demand release
dc.subjectPh-responsive
dc.subjectsynergistic therapy
dc.titleAn on-demand antibacterial hydrogel for precise and rapid elimination of bacterial infection in a murine partial thickness scald burn wound
dc.typeJournal article
pubs.publication-statusPublished
ror.fileinfo12286940050001831 13286940040001831 An on-demand antibacterial hydrogel for precise and rapid elimination of bacterial infection in a murine partial thickness scald burn wound
ror.mmsid9916862324001831

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