Extracorporeal normothermic pancreas perfusion

dc.contributor.advisorMaddern, Guy John
dc.contributor.advisorTrochsler, Markus
dc.contributor.authorKuan, Kean Guan
dc.contributor.schoolSchool of Medicineen
dc.date.issued2016
dc.description.abstractPancreas and islet transplantation are important treatment options for insulin dependent diabetes. However, one of the main challenges in pancreas and islet transplantation lies in organ (pancreas) preservation. Ischaemic injury post-retrieval causes significant damage the organ function and reduction in islet yield. It was hypothesised that extracorporeal normothermic perfusion of the pancreas would improve graft function post transplantation and pancreatic islet isolation when compared to traditional methods of organ preservation. The aims and objectives of the project included: • Conducting a systematic review of the literature in extracorporeal machine perfusion of the pancreas • Establishing an extracorporeal normothermic perfusion model of the porcine pancreas • Comparing the addition of kidney to the circuit of extracorporeal pancreas perfusion model A thorough systematic review of the available literature concluded the potential benefits of machine perfusion in pancreas preservation. Important insight into the experimental setup, perfusion parameters and vital outcome measures was also attained. A model of normothermic hemo-perfusion of the porcine pancreas with and without addition of the kidney as a dialysis organ was subsequently established. The organs were perfused for 120 minutes with stable perfusion parameters but sub-optimal acid-base environment. Addition of the kidney did not result in significant improvement of the acid-base environment.en
dc.description.dissertationThesis (M.Phil.) (Research by Publication) -- University of Adelaide, School of Medicine, 2016.en
dc.identifier.doi10.4225/55/583501688ead6
dc.identifier.urihttp://hdl.handle.net/2440/102708
dc.provenanceThis electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exceptions. If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at: http://www.adelaide.edu.au/legalsen
dc.subjectextracorporeal perfusionen
dc.subjectex-vivo perfusionen
dc.subjectnormothermic perfusionen
dc.subjectpancreasen
dc.titleExtracorporeal normothermic pancreas perfusionen
dc.typeThesesen

Files

Original bundle
Now showing 1 - 3 of 3
No Thumbnail Available
Name:
01front.pdf
Size:
3.88 MB
Format:
Adobe Portable Document Format
No Thumbnail Available
Name:
02whole.pdf
Size:
17.23 MB
Format:
Adobe Portable Document Format
No Thumbnail Available
Name:
Restricted
Size:
11.69 MB
Format:
Adobe Portable Document Format
Description:
Library staff access only

Collections