A genomic perspective of metal-resistant bacteria from gold particles: Possible survival mechanisms during gold biogeochemical cycling

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dc.contributor.authorSanyal, S.K.
dc.contributor.authorReith, F.
dc.contributor.authorShuster, J.
dc.date.issued2020
dc.descriptionAdvance Access Publication Date: 4 June 2020.
dc.description.abstractA bacterial consortium was enriched from gold particles that 'experienced' ca. 80 years of biotransformation within waste-rock piles (Australia). This bacterial consortium was exposed to 10 µM AuCl3 to obtain Au-tolerant bacteria. From these isolates, Serratia sp. and Stenotrophomonas sp. were the most Au-tolerant and reduced soluble Au as pure gold nanoparticles, indicating that passive mineralisation is a mechanism for mediating the toxic effect of soluble Au produced during particle dissolution. Genome-wide analysis demonstrated that these isolates also possessed various genes that could provide cellular defence enabling survival under heavy-metal stressed condition by mediating the toxicity of heavy metals through active efflux/reduction. Diverse metal-resistant genes or genes clusters (cop, cus, czc, znt, ars) were detected, which could confer resistance to soluble Au. Comparative genome analysis revealed that the majority of detected heavy-metal resistant genes were similar (i.e. orthologous) to those genes of Cupriavidus metallidurans CH34. The detection of heavy-metal resistance, nutrient cycling, and biofilm formation genes (pgaABCD, bsmA, hmpS) may have indirect yet important roles when dealing with soluble Au during particle dissolution. In conclusion, the physiological and genomic results suggest that bacteria living on gold particles would likely use various genes to ensure survival during Au biogeochemical cycling.
dc.description.statementofresponsibilitySantonu Kumar Sanyal, Frank Reith and Jeremiah Shuster
dc.identifier.citationFEMS Microbiology Ecology, 2020; 96(7):fiaa111-1-fiaa111-15
dc.identifier.doi10.1093/femsec/fiaa111
dc.identifier.issn0168-6496
dc.identifier.issn1574-6941
dc.identifier.orcidSanyal, S.K. [0000-0001-5049-4297]
dc.identifier.orcidShuster, J. [0000-0002-9839-6618]
dc.identifier.urihttp://hdl.handle.net/2440/126288
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.relation.granthttp://purl.org/au-research/grants/arc/FT150100250
dc.rights© FEMS 2020. All rights reserved.
dc.source.urihttps://doi.org/10.1093/femsec/fiaa111
dc.subjectGold particles
dc.subjectHeavy-metal resistance
dc.subjectSerratia
dc.subjectStenotrophomonas
dc.subjectGold biogeochemistry
dc.subjectGold biogeochemical cycling
dc.subjectAu-tolerant bacteria
dc.titleA genomic perspective of metal-resistant bacteria from gold particles: Possible survival mechanisms during gold biogeochemical cycling
dc.typeJournal article
pubs.publication-statusPublished

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