Development and characterization of 13 new, and cross amplification of 3, polymorphic nuclear microsatellite loci in the common myna (Acridotheres tristis)
Date
2012
Authors
Berthouly-Salazar, C.
Cassey, P.
van Vuuren, B.
van Rensburg, B.
Hui, C.
Gardner, M.
Le Roux, J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Conservation Genetics Resources, 2012; 4(3):621-624
Statement of Responsibility
Cécile Berthouly-Salazar, Phillip Cassey, Bettine Jansen van Vuuren, Berndt Janse van Rensburg, Cang Hui, Michael G. Gardner, Johannes J. Le Roux
Conference Name
Abstract
We used a next generation sequencing (NGS) approach to screen for genome-wide nuclear microsatellite loci in the common (Indian) myna, Acridotheres tristis. In addition, markers previously developed for other Sturnidae species were tested for cross-amplification in A. tristis. In total, we identified 20 loci from NGS data and tested 26 loci for cross-amplification. Out of all loci (NGS developed and cross-amplified), 16 unlinked loci showed polymorphism, ranging from 2 to 9 alleles per locus. Test individuals were obtained from the invasive ranges of common myna in South Africa. Overall, expected and observed heterozygosities ranged from 0.089 to 0.802 and from 0.094 to 0.906, respectively. These markers will be used to shed light on invasion genetics and landscape-scale dynamics of invasive A. tristis in South Africa. © 2012 Springer Science+Business Media B.V.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© Springer Science+Business Media B.V. 2012