Identification and partial characterization of glycosidic activities of commercial strains of the lactic acid bacterium, Oenococcus oeni
| dc.contributor.author | Grimaldi, A. | |
| dc.contributor.author | McLean, H. | |
| dc.contributor.author | Jiranek, V. | |
| dc.date.issued | 2000 | |
| dc.description.abstract | β-glucosidase activity was measured in cultures derived from 11 commercial preparations of Oenococcus oeni, which are utilized for the malolactic fermentation of wine. All cultures produced a detectable activity seen as the hydrolysis of p-nitrophenyl-ß-D-glucopyranoside. Detectable activities against p-nitrophenyl-ß-D-xylopyranoside and, to a lesser extent for selected isolates, p-nitrophenyl-α-L-arabinopyranoside, were also observed. The activity occurred in both the supernatant and cell-associated fractions, with the combined total activity and its relative distribution varying according to strain and growth stage. In evaluating the potential of these activities as liberators of glyco-conjugated grape aromas, responses to enological pH values, glucose/ fructose and ethanol concentrations were determined. Over the pH range of 3.5 to 4.0, 12% to 43% of the activity observed at the optimal pH of 5.5 was retained. Depending on the isolate examined, the presence of up to 166 mM sugar yielded responses ranging between 30% enhancement and 93% inhibition. Similarly, responses to ethanol ranged from 80% enhancement to 73% inhibition. When examined in a combinatorial manner, a heightened inhibition by some pH/sugar/ethanol combinations indicated a synergism for some variables. The potential enological significance of these findings is discussed. | |
| dc.description.statementofresponsibility | Antonio Grimaldi, Hylton McLean and Vladimir Jiranek | |
| dc.identifier.citation | American Journal of Enology and Viticulture, 2000; 51(4):362-369 | |
| dc.identifier.doi | 10.5344/ajev.2000.51.4.362 | |
| dc.identifier.issn | 0002-9254 | |
| dc.identifier.issn | 1943-7749 | |
| dc.identifier.orcid | Jiranek, V. [0000-0002-9775-8963] | |
| dc.identifier.uri | http://hdl.handle.net/2440/32459 | |
| dc.language.iso | en | |
| dc.publisher | American Society of Enologists and Viticulture | |
| dc.rights | © 2000 by the American Society for Enology and Viticulture | |
| dc.source.uri | http://www.ajevonline.org/content/51/4/362.full.pdf+html | |
| dc.title | Identification and partial characterization of glycosidic activities of commercial strains of the lactic acid bacterium, Oenococcus oeni | |
| dc.type | Journal article | |
| pubs.publication-status | Published |