MALDI mass spectrometry imaging of N-glycans on tibial cartilage and subchondral bone proteins in knee osteoarthritis
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(Accepted version)
Date
2016
Authors
Briggs, M.
Kuliwaba, J.
Muratovic, D.
Everest-Dass, A.
Packer, N.
Findlay, D.
Hoffmann, P.
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Journal article
Citation
Proteomics, 2016; 16(11-12):1736-1741
Statement of Responsibility
Matthew T. Briggs, Julia S. Kuliwaba, Dzenita Muratovic, Arun V. Everest, Dass, Nicolle H. Packer, David M. Findlay, Peter Hoffmann
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Abstract
Magnetic Resonance Imaging (MRI) is a non-invasive technique routinely used to investigate pathological changes in knee osteoarthritis (OA) patients. MRI uniquely reveals zones of the most severe change in the subchondral bone (SCB) in OA, called bone marrow lesions (BMLs). BMLs have diagnostic and prognostic significance in OA, but MRI does not provide a molecular understanding of BMLs. Multiple N-glycan structures have been observed to play a pivotal role in the OA disease process. We applied matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) of N-glycans to formalin-fixed paraffin-embedded (FFPE) SCB tissue sections from patients with knee OA, and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) was conducted on consecutive sections to structurally characterize and correlate with the N-glycans seen by MALDI-MSI. The application of this novel MALDI-MSI protocol has enabled the first steps to spatially investigate the N-glycome in the SCB of knee OA patients.
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Accepted manuscript online: 15 March 2016
Link to a related website: https://rss.onlinelibrary.wiley.com/doi/am-pdf/10.1002/pmic.201500461, Open Access via Unpaywall
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© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim