A Drosophila overexpression screen for modifiers of Rho signalling in cytokinesis

Simple item page
dc.contributor.authorGregory, S.
dc.contributor.authorShandala, T.
dc.contributor.authorO'Keefe, L.
dc.contributor.authorJones, L.
dc.contributor.authorMurray, M.
dc.contributor.authorSaint, R.
dc.contributor.organisationCentre for the Molecular Genetics of Development
dc.date.issued2007
dc.description© 2000-2008 Landes Bioscience
dc.description.abstractTo identify genes that modulate Rho signalling during cytokinesis we tested the effect of overexpressing a set of 2190 genes on an eye phenotype caused by defective Rho activation. The resulting 112 modifier loci fell into three main classes: cell cycle genes, signalling effectors and metabolic enzymes. We developed a further series of genetic tests to refine the interactors into those most likely to modify Rho signalling during cytokinesis. In addition to a number of genes previously implicated in the Rho pathway during cytokinesis, we identified four novel primary candidates: cdc14, Pitslre, PDK1 and thread/diap1. cdc14 orthologs have, however, been implicated in cytokinesis in other organisms, as have molecules related to Thread/Diap1. The identification of several modifiers that are genetically redundant paralogs highlights the ability of overexpression screens to identify genes that are refractory to traditional loss-of-function approaches. Overexpression screens and sensitized phenotypes, therefore, may help identify the many factors that are expected to be involved in cytokinesis but have not been discovered by previous genetic screens.
dc.description.statementofresponsibilityStephen L. Gregory, Tetyana Shandala, Louise O'Keefe, Lynn Jones, Michael J. Murray and Robert Saint
dc.identifier.citationFly, 2007; 1(1):13-23
dc.identifier.doi10.4161/fly.3806
dc.identifier.issn1933-6934
dc.identifier.issn1933-6942
dc.identifier.orcidGregory, S. [0000-0002-0046-5815]
dc.identifier.urihttp://hdl.handle.net/2440/43298
dc.language.isoen
dc.publisherLandes Bioscience
dc.source.urihttp://www.landesbioscience.com/journals/fly/article/3806
dc.subjectEye
dc.subjectAnimals
dc.subjectDrosophila
dc.subjectrhoA GTP-Binding Protein
dc.subjectGuanine Nucleotide Exchange Factors
dc.subjectDrosophila Proteins
dc.subjectGenetic Markers
dc.subjectSignal Transduction
dc.subjectCytokinesis
dc.subjectRNA Interference
dc.subjectWings, Animal
dc.titleA Drosophila overexpression screen for modifiers of Rho signalling in cytokinesis
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Molecular and Biomedical Science publications
Centre for the Molecular Genetics of Development publications