A monophyletic origin of the B chromosomes of Brachycome dichromosomatica (Asteraceae)

Date

1999

Authors

Houben, A.
Thompson, N.
Ahne, R.
Leach, C.
Verlin, D.
Timmis, J.

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Plant Systematics and Evolution, 1999; 219(1):127-135

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Houben, Andreas ; Thompson, Nicole ; Ahne, Richard ; Leach, Carolyn ; Verlin, Dawn ; Timmis, Jeremy

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Abstract

The A and B chromosomes of different karyotype variants(cytodemes A1, A2, A3 and A4) of Brachycome dichromosomatica were analysed by computer-aided chromosome image analysis and fluorescence in situ hybridisation (FISH). Ribosomal DNA and the B chromosome-specific sequence Bd49 were detected on all B chromosomes. In addition to minor size variation of the Bs, polymorphism of the rDNA and Bd49 position and copy number revealed two major types of B chromosomes. The B chromosomes of all the cytodemes were indistinguishable from each other in length, but that of A3 showed evidence of rearrangements consistent with its long-term geographic isolation. The results presented suggest a monophyletic origin of the B chromosomes of B. dichromosomatica.

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