Molecular evolution of glutathione S-transferases in the genus Drosophila

Simple item page
dc.contributor.authorLow, W.Y.
dc.contributor.authorNg, H.L.
dc.contributor.authorMorton, C.J.
dc.contributor.authorParker, M.W.
dc.contributor.authorBatterham, P.
dc.contributor.authorRobin, C.
dc.date.issued2007
dc.description.abstractAs classical phase II detoxification enzymes, glutathione S-transferases (GSTs) have been implicated in insecticide resistance and may have evolved in response to toxins in the niche-defining feeding substrates of Drosophila species. We have annotated the GST genes of the 12 Drosophila species with recently sequenced genomes and analyzed their molecular evolution. Gene copy number variation is attributable mainly to unequal crossing-over events in the large d and e clusters. Within these gene clusters there are also GST genes with slowly diverging orthologs. This implies that they have their own unique functions or have spatial/temporal expression patterns that impose significant selective constraints. Searches for positively selected sites within the GSTs identified G171K in GSTD1, a protein that has previously been shown to be capable of metabolizing the insecticide DDT. We find that the same radical substitution (G171K) in the substrate-binding domain has occurred at least three times in the Drosophila radiation. Homology-modeling places site 171 distant from the active site but adjacent to an alternative DDT-binding site. We propose that the parallel evolution observed at this site is an adaptive response to an environmental toxin and that sequencing of historical alleles suggests that this toxin was not a synthetic insecticide.
dc.description.statementofresponsibilityWai Yee Low, Hooi Ling Ng, Craig J. Morton, Michael W. Parker, Philip Batterham and Charles Robin
dc.identifier.citationGenetics, 2007; 177(3):1363-1375
dc.identifier.doi10.1534/genetics.107.075838
dc.identifier.issn0016-6731
dc.identifier.issn1943-2631
dc.identifier.orcidLow, W.Y. [0000-0002-0749-765X]
dc.identifier.urihttp://hdl.handle.net/2440/116059
dc.language.isoen
dc.publisherThe Genetics Society of America
dc.relation.granthttp://purl.org/au-research/grants/arc/DP0557497
dc.rightsCopyright © 2007 by the Genetics Society of America
dc.source.urihttps://doi.org/10.1534/genetics.107.075838
dc.subjectAnimals
dc.subjectDrosophila
dc.subjectDDT
dc.subjectGlutathione Transferase
dc.subjectDrosophila Proteins
dc.subjectDNA Primers
dc.subjectInsecticides
dc.subjectEvolution, Molecular
dc.subjectPhylogeny
dc.subjectSpecies Specificity
dc.subjectGene Duplication
dc.subjectGene Deletion
dc.subjectBase Sequence
dc.subjectInsecticide Resistance
dc.subjectGenes, Insect
dc.subjectMultigene Family
dc.subjectModels, Genetic
dc.subjectModels, Molecular
dc.subjectSelection, Genetic
dc.titleMolecular evolution of glutathione S-transferases in the genus Drosophila
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Animal and Veterinary Sciences publications