Characterization and PCR multiplexing of polymorphic microsatellite loci in cashew (Anacardium occidentale L.) and their cross-species utilization

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dc.contributor.authorCroxford, A.
dc.contributor.authorRobson, M.
dc.contributor.authorWilkinson, M.
dc.date.issued2006
dc.description.abstractCashew (Anacardium occidentale L.) is the most economically important tropical nut crop in the world, and yet there are no sequence tagged site (STS) markers available for its study. Here we use an automated, high-throughput system to isolate cashew microsatellites from a non-enriched genomic library blotted onto membranes at high density for screening. Sixty-five sequences contained a microsatellite array, of which 21 proved polymorphic among a closely related seed garden population of 49 genotypes. Twelve markers were suitable for multiplex analysis. Of these, 10 amplified in all three related tropical tree species tested: Anacardium microcarpum, Anacardium pumilum and Anacardium nanum.
dc.description.statementofresponsibilityA. E. Croxford, M. Robson and M. J . Wilkinson
dc.identifier.citationMolecular Ecology Resources, 2006; 6(1):249-251
dc.identifier.doi10.1111/j.1471-8286.2005.01208.x
dc.identifier.issn1471-8286
dc.identifier.issn1471-8286
dc.identifier.urihttp://hdl.handle.net/2440/70515
dc.language.isoen
dc.publisherBlackwell Publishing Ltd.
dc.rights© 2006 Blackwell Publishing Ltd
dc.source.urihttps://doi.org/10.1111/j.1471-8286.2005.01208.x
dc.subjectAnacardium
dc.subjectcashew
dc.subjectcross-amplification
dc.subjectmicrosatellites
dc.subjectpolymorphism
dc.titleCharacterization and PCR multiplexing of polymorphic microsatellite loci in cashew (Anacardium occidentale L.) and their cross-species utilization
dc.typeJournal article
pubs.publication-statusPublished

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