Inhibition of low-density lipoprotein oxidation and up-regulation of low-density lipoprotein receptor in HepG2 cells by tropical plant extracts
| dc.contributor.author | Salleh, Mohammed Nizar | en |
| dc.contributor.author | Runnie, Irine | en |
| dc.contributor.author | Roach, Paul D. | en |
| dc.contributor.author | Mohamed, Suhaila | en |
| dc.contributor.author | Abeywardena, Mahinda Y. | en |
| dc.date.issued | 2002 | en |
| dc.description.abstract | Twelve edible plant extracts rich in polyphenols were screened for their potential to inhibit oxidation of low-density lipoprotein (LDL) in vitro and to modulate LDL receptor (LDLr) activity in cultured HepG2 cells. The antioxidant activity (inhibition of LDL oxidation) was determined by measuring the formation of conjugated dienes (lag time) and thiobarbituric acid reagent substances (TBARS). Betel leaf (94%), cashew shoot (63%), Japanese mint (52%), semambu leaf (50%), palm frond (41%), sweet potato shoot, chilli fruit, papaya shoot, roselle calyx, and maman showed significantly increased lag time (>55 min, P < 0.05) and inhibition of TBARS formation (P < 0.05) compared to control. LDLr was significantly up-regulated (P < 0.05) by Japanese mint (67%), semambu (51%), cashew (50%), and noni (49%). Except for noni and betel leaf, most plant extracts studied demonstrated a positive association between antioxidant activity and the ability to up-regulate LDL receptor. Findings suggest that reported protective actions of plant polyphenols on lipoprotein metabolism might be exerted at different biochemical mechanisms. | en |
| dc.description.statementofresponsibility | Mohd. Nizar Salleh, Irine Runnie, Paul D. Roach, Suhaila Mohamed and Mahinda Y. Abeywardena | en |
| dc.identifier.citation | Journal of Agricultural and Food Chemistry, 2002; 50(13):3693-3697 | en |
| dc.identifier.doi | 10.1021/jf011593f | en |
| dc.identifier.issn | 0021-8561 | en |
| dc.identifier.uri | http://hdl.handle.net/2440/14453 | |
| dc.language.iso | en | en |
| dc.publisher | American Chemical Society | en |
| dc.rights | Copyright © 2002 American Chemical Society | en |
| dc.subject | LDL oxidation ; LDL receptor ; plant extracts ; TBARS ; HepG2 cells | en |
| dc.title | Inhibition of low-density lipoprotein oxidation and up-regulation of low-density lipoprotein receptor in HepG2 cells by tropical plant extracts | en |
| dc.type | Journal article | en |