Cryopreservation of in vitro almond shoot tips by vitrification
| dc.contributor.author | Channuntapipat, C. | |
| dc.contributor.author | Collins, G. | |
| dc.contributor.author | Bertozzi, T. | |
| dc.contributor.author | Sedgley, M. | |
| dc.date.issued | 2000 | |
| dc.description.abstract | Shoot tips of two almond scion cultivars, 'Ne Plus Ultra' and 'Nonpareil 15-1', and one almond/peach hybrid rootstock were successfully cryopreserved using a one-step vitrification technique. Three week old in vitro cultures were cold-hardened at 4°C on the multiplication medium (Murashige and Skoog for 'Ne Plus Ultra' and the hybrid rootstock; Almehdi and Parfitt for 'Nonpareil 15-1') for three weeks. Shoot tips, 2-2.5 mm long, were excised and precultured for 1 d at 4°C on the same basal medium, without plant growth regulators, supplemented with 0.7 M sucrose. After the preculture, the shoot tips were incubated in vitrification solution at 25°C for 45 min for the almond scion cultivars and 160 min for the hybrid rootstock, and then stored under liquid nitrogen (LN) for at least 3 d. After rapid thawing at 30°C, the shoot tips were washed with the appropriate liquid basal medium containing 1.0 M sucrose and then cultured on the same basal medium, solidified with agar, but excluding NH<inf>4</inf>NO<inf>3</inf> or (NH<inf>4</inf>)<inf>2</inf>SO<inf>4</inf>. Shoot regeneration was usually observed within 2-3 weeks. Survival after LN, recorded as the percentage of shoot tips that produced at least one new shoot four weeks after thawing, was 87.5, 60.0 and 72.5% for 'Ne Plus Ultra', 'Nonpareil 15-1' and the hybrid rootstock respectively. The one-step vitrification method is a promising simple technique for cryopreserving almond scion and rootstock shoot tips from in vitro cultures. | |
| dc.description.uri | http://www.jhortscib.org/Vol75/75_2/17.htm | |
| dc.identifier.citation | Journal of Horticultural Science and Biotechnology, 2000; 75(2):228-232 | |
| dc.identifier.doi | 10.1080/14620316.2000.11511228 | |
| dc.identifier.issn | 1462-0316 | |
| dc.identifier.issn | 2380-4084 | |
| dc.identifier.orcid | Bertozzi, T. [0000-0001-6665-3395] | |
| dc.identifier.uri | http://hdl.handle.net/2440/13062 | |
| dc.language.iso | en | |
| dc.publisher | Headley Brothers Ltd | |
| dc.source.uri | https://doi.org/10.1080/14620316.2000.11511228 | |
| dc.title | Cryopreservation of in vitro almond shoot tips by vitrification | |
| dc.type | Journal article | |
| pubs.publication-status | Published |