A cytochrome P450 class I electron transfer system from Novosphingobium aromaticivorans

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dc.contributor.authorBell, S.
dc.contributor.authorDale, A.
dc.contributor.authorRees, N.
dc.contributor.authorWong, L.
dc.date.issued2010
dc.description.abstractCytochrome P450 (CYP) enzymes of the CYP101 and CYP111 families from Novosphingobium aromaticivorans are heme monooxygenases that catalyze the hydroxylation of a range of terpenoid compounds. CYP101D1 and CYP101D2 oxidized camphor to 5-exo-hydroxycamphor. CYP101B1 and CYP101C1 oxidized beta-ionone to predominantly 3-R-hydroxy-beta-ionone and 4-hydroxy-beta-ionone, respectively. CYP111A2 oxidized linalool to 8-hydroxylinalool. Physiologically, these CYP enzymes could receive electrons from Arx, a [2Fe-2S] ferredoxin equivalent to putidaredoxin from the CYP101A1 system from Pseudomonas putida. A putative ferredoxin reductase (ArR) in the N. aromaticivorans genome, with high amino acid sequence homology to putidaredoxin reductase, has been over-produced in Escherichia coli and found to support substrate oxidation by these CYP enzymes via Arx with both high activity and coupling of product formation to NADH consumption. The ArR/Arx electron-transport chain has been co-expressed with the CYP enzymes in an E. coli host to provide in vivo whole-cell substrate oxidation systems that could produce up to 6.0 g L(-1) of 5-exo-hydroxycamphor at rates of up to 64 microM (gram of cell dry weight)(-1) min(-1). These efficient biocatalytic systems have potential uses in preparative scale whole-cell biotransformations.
dc.description.statementofresponsibilityStephen G. Bell, Alison Dale, Nicholas H. Rees, Luet-Lok Wong
dc.identifier.citationApplied Microbiology and Biotechnology, 2010; 86(1):163-175
dc.identifier.doi10.1007/s00253-009-2234-y
dc.identifier.issn0175-7598
dc.identifier.issn1432-0614
dc.identifier.orcidBell, S. [0000-0002-7457-9727]
dc.identifier.urihttp://hdl.handle.net/2440/75177
dc.language.isoen
dc.publisherSpringer-Verlag
dc.rights© Springer-Verlag 2009
dc.source.urihttps://doi.org/10.1007/s00253-009-2234-y
dc.subjectCytochrome P450
dc.subjectNovosphingobium aromaticivorans
dc.subjectElectron transfer
dc.subjectFerredoxin reductase
dc.subjectWhole-cell biotransformations
dc.titleA cytochrome P450 class I electron transfer system from Novosphingobium aromaticivorans
dc.typeJournal article
pubs.publication-statusPublished

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