Expression of defensin antimicrobial peptides in the peritoneal cavity of patients on peritoneal dialysis

Simple item page
dc.contributor.authorZarrinkalam, K.
dc.contributor.authorLeavesley, D.
dc.contributor.authorStanley, J.
dc.contributor.authorAtkins, G.
dc.contributor.authorFaull, R.
dc.date.issued2001
dc.descriptionCopyright © 2001 by International Society for Peritoneal Dialysis
dc.description.abstractOBJECTIVE: To investigate the expression and regulation of defensins in the peritoneal cavity of peritoneal dialysis (PD) patients. DESIGN: The presence of defensins in the peritoneal cavity was assessed using reverse transcription polymerase chain reaction (RT-PCR). In vivo defensin expression was analyzed in human peritoneal membrane biopsies and in peritoneal cavity leukocytes isolated from spent dialysate. Defensin expression in vitro was assessed in cultured human peritoneal mesothelial cells (HPMC) and confirmed with PCR Southern blot and DNA sequencing. The effect of tumor necrosis factor alpha (TNFalpha) and epidermal growth factor (EGF) on beta2 defensin expression in HPMC was analyzed by Northern blot analysis and RT-PCR respectively. RESULTS: Both alpha and beta classes of defensins are expressed in the peritoneal cavity of PD patients. Messenger RNA for the alpha-defensin human neutrophil peptide 3 and for beta-defensin-1 (hbetaD-1) were found in preparations containing predominantly peritoneal leukocytes, whereas beta-defensin-2 (hbetaD-2) is expressed by HPMC. HPMC isolated from different individuals displayed variability in both basal hbetaD-2 expression and in response to stimulation by TNFalpha. Conversely, EGF consistently downregulated the level of hbetaD-2 message in HPMC. CONCLUSION: Alpha- and beta-defensins are expressed in the peritoneal cavity, and hbetaD-2 is the main defensin present in the peritoneal membrane. Variable levels of expression of hbetaD-2 by mesothelial cells were seen, with evidence of regulation by cytokines and growth factors. This provides evidence for a previously unknown mechanism of innate immunity at that site.
dc.description.statementofresponsibilityKH Zarrinkalam, DI Leavesley, JM Stanley, GJ Atkins, and RJ Faull
dc.identifier.citationPeritoneal Dialysis International, 2001; 21(5):501-508
dc.identifier.doi10.1177/089686080102100512
dc.identifier.issn0896-8608
dc.identifier.issn1718-4304
dc.identifier.orcidAtkins, G. [0000-0002-3123-9861]
dc.identifier.urihttp://hdl.handle.net/2440/9744
dc.language.isoen
dc.publisherMultimed Inc
dc.source.urihttp://pdiconnect.com/cgi/content/abstract/21/5/501
dc.subjectPeritoneal Cavity
dc.subjectCells, Cultured
dc.subjectEpithelial Cells
dc.subjectHumans
dc.subjectPeritonitis
dc.subjectEpidermal Growth Factor
dc.subjectalpha-Defensins
dc.subjectbeta-Defensins
dc.subjectTumor Necrosis Factor-alpha
dc.subjectRNA, Messenger
dc.subjectPeritoneal Dialysis
dc.subjectBlotting, Northern
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSequence Analysis, DNA
dc.subjectBase Sequence
dc.subjectMolecular Sequence Data
dc.titleExpression of defensin antimicrobial peptides in the peritoneal cavity of patients on peritoneal dialysis
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Medicine publications