Lysosomal LAMP1 immunoreactivity exists in both diffuse and neuritic amyloid plaques in the human hippocampus
Date
2018
Authors
Hassiotis, S.
Manavis, J.
Blumbergs, P.C.
Hattersley, K.J.
Carosi, J.M.
Kamei, M.
Sargeant, T.J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
European Journal of Neuroscience, 2018; 47(9):1043-1053
Statement of Responsibility
Sofia Hassiotis, Jim Manavis, Peter C. Blumbergs, Kathryn J. Hattersley, Julian M. Carosi, Makoto Kamei, and Timothy J. Sargeant
Conference Name
Abstract
Lysosomal vesicles around neuritic plaques are thought to drive Alzheimer's disease by providing ideal microenvironments for generation of amyloid-β. Although lysosomal vesicles are present at every amyloid plaque in mouse models of Alzheimer's disease, the number of amyloid plaques that contain lysosomal vesicles in the human brain remains unknown. This study aimed to quantify lysosomal vesicles at amyloid plaques in the human hippocampus. Lysosome-associated membrane protein 1 (LAMP1)-positive vesicles accumulated in both diffuse (Aβ42-positive/AT8-negative) and neuritic (Aβ42-positive/AT8-positive) plaques in all regions were analysed. In contrast to mouse models of Alzheimer's disease, however, not all amyloid plaques accumulated LAMP1-positive lysosomal vesicles. Even at neuritic plaques, LAMP1 immunoreactivity was more abundant than phospho-tau (AT8). Further, lysosomal vesicles colocalised weakly with phospho-tau such that accumulation of lysosomal vesicles and phospho-tau appeared to be spatially distinct events that occurred within dystrophic neurites. This quantitative study shows that diffuse plaques, as well as neuritic plaques, contain LAMP1 immunoreactivity in the human hippocampus.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© 2018 Federation of European Neuroscience Societies and John Wiley & Sons Ltd