Identification of a novel group of putative Arabidopsis thaliana β-(1,3)-galactosyltransferases

Simple item page
dc.contributor.authorQu, Y.
dc.contributor.authorEgelund, J.
dc.contributor.authorGilson, P.
dc.contributor.authorHoughton, F.
dc.contributor.authorGleeson, P.
dc.contributor.authorSchultz, C.
dc.contributor.authorBacic, A.
dc.date.issued2008
dc.descriptionThe original publication can be found at www.springerlink.com
dc.description.abstractTo begin biochemical and molecular studies on the biosynthesis of the type II arabinogalactan chains on arabinogalactan-proteins (AGPs), we adopted a bioinformatic approach to identify and systematically characterise the putative galactosyltransferases (GalTs) responsible for synthesizing the β-(1,3)-Gal linkage from CAZy GT-family-31 from Arabidopsis thaliana. These analyses confirmed that 20 members of the GT-31 family contained domains/motifs typical of biochemically characterised β-(1,3)-GTs from mammalian systems. Microarray data confirm that members of this family are expressed throughout all tissues making them likely candidates for the assembly of the ubiquitously found AGPs. One member, At1g77810, was selected for further analysis including location studies that confirmed its presence in the Golgi and preliminary enzyme substrate specificity studies that demonstrated β-(1,3)-GalT activity. This bioinformatic/molecular study of CAZy GT-family-31 was validated by the recent report of Strasser et al. (Plant Cell 19:2278–2292, 2007) that another member of this family (At1g26810; GALT1) encodes a β-(1,3)-GalT involved in the biosynthesis of the Lewis a epitope of N-glycans in Arabidopsis thaliana.
dc.description.statementofresponsibilityYongmei Qu, Jack Egelund, Paul R. Gilson, Fiona Houghton, Paul A. Gleeson, Carolyn J. Schultz and Antony Bacic
dc.identifier.citationPlant Molecular Biology: An International Journal on Molecular Biology, Molecular Genetics and Biochemistry, 2008; 68(1-2):43-59
dc.identifier.doi10.1007/s11103-008-9351-3
dc.identifier.issn0167-4412
dc.identifier.issn1573-5028
dc.identifier.urihttp://hdl.handle.net/2440/53246
dc.language.isoen
dc.publisherKluwer Academic Publ
dc.source.urihttps://doi.org/10.1007/s11103-008-9351-3
dc.subjectArabinogalactan-protein
dc.subjectCAZy-database
dc.subjectGalactosyltransferase
dc.subjectGlycosyltransferase
dc.subjectGolgi
dc.titleIdentification of a novel group of putative Arabidopsis thaliana β-(1,3)-galactosyltransferases
dc.title.alternativeIdentification of a novel group of putative Arabidopsis thaliana beta-(1,3)-galactosyltransferases
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Agriculture, Food and Wine publications