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Fast and highly sensitive detection of pathogens wreathed with magnetic nanoparticles using dark-field microscopy

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dc.contributor.authorChen, F.
dc.contributor.authorTang, F.
dc.contributor.authorYang, C.T.
dc.contributor.authorZhao, X.
dc.contributor.authorWang, J.
dc.contributor.authorThierry, B.
dc.contributor.authorBansal, V.
dc.contributor.authorDai, J.
dc.contributor.authorZhou, X.
dc.date.issued2018
dc.description.abstractCryptosporidium parvum (C. parvum) is a highly potent zoonotic pathogen, which can do significant harm to both human beings and livestock. However, existing technologies or methods are deficient for rapid on-site detection of water contaminated with C. parvum. Better detection approaches are needed to allow water management agencies to stop major breakouts of the pathogen. Herein, we present a novel detection method for cryptosporidium in a tiny drop of sample using a magnetic nanoparticle (MNP) probe combined with dark-field microscopy in 30 min. The designed MNP probes bind with high affinity to C. parvum, resulting in the formation of a golden garland-like structure under dark-field microscopy. This MNP-based dark-field counting strategy yields an amazing PCR-like sensitivity of 8 attomolar (aM) (5 pathogens in 1 μL). Importantly, the assay is very rapid (∼30 min) and is very simple to perform as it involves only one step of mixing and magnetic separation, followed by dropping on a slide for counting under dark-field microscope. By combining the advantages of the specific light-scattering characteristic of MNP probe under dark field and the selective magnetic separation ability of functionalized MNP, the proposed MNP-based dark-field enumeration method offers low cost and significant translational potential.
dc.identifier.citationACS Sensors, 2018; 3(10):2175-2181
dc.identifier.doi10.1021/acssensors.8b00785
dc.identifier.issn2379-3694
dc.identifier.issn2379-3694
dc.identifier.urihttps://hdl.handle.net/11541.2/134766
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.fundingARC 140101285
dc.rightsCopyright 2018 American Chemical Society Access Condition Notes: Accepted manuscript available after 1 October 2019
dc.source.urihttps://doi.org/10.1021/acssensors.8b00785
dc.subjectcryptosporidium parvum
dc.subjectdark-field microscope
dc.subjectgarland-like structure
dc.subjectmagnetic nanoparticles
dc.subjectpathogens detection
dc.titleFast and highly sensitive detection of pathogens wreathed with magnetic nanoparticles using dark-field microscopy
dc.typeJournal article
pubs.publication-statusPublished
ror.fileinfo12167112080001831 13167112070001831 9916237798601831pp.pdf
ror.mmsid9916237798601831

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