An essential function for the centrosomal protein NEDD1 in zebrafish development

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dc.contributor.authorManning, J.
dc.contributor.authorLewis, M.
dc.contributor.authorKoblar, S.
dc.contributor.authorKumar, S.
dc.date.issued2010
dc.description.abstractThe centrosome is the primary microtubule organising centre of the cell. It is composed of many proteins, some of which make up the core of the centrosome, whereas others are used for specific functions. Although the cellular roles of many centrosomal proteins are well defined, much less is known about their functions and the role of the centrosome in development. In this study we investigated the function of NEDD1, a critical component of the centrosome essential for microtubule nucleation, in zebrafish (Danio rerio) development. The zebrafish homologue of NEDD1 (zNEDD1) was cloned and found to have a similar localisation and function to mammalian NEDD1. We show that zNEDD1 is essential for survival, as a high level of knockdown was embryonic lethal. Partial knockdown of zNEDD1 caused abnormalities including an increase in mitotic and apoptotic cells. Pronounced phenotypic defects were seen in the brain, with a lack of defined brain structures, incomplete neural tube formation and a disorganisation of neurons. In addition, we show that a reduction in zNEDD1 resulted in the loss of c-tubulin at the centrosome. Our data thus demonstrate that zNEDD1 is critical for the recruitment of c-tubulin to the centrosome, and is essential for the proper development of zebrafish.
dc.description.statementofresponsibilityJA Manning, M Lewis, SA Koblar and S Kumar
dc.identifier.citationCell Death and Differentiation, 2010; 17(8):1302-1314
dc.identifier.doi10.1038/cdd.2010.12
dc.identifier.issn1350-9047
dc.identifier.issn1476-5403
dc.identifier.orcidLewis, M. [0000-0002-3332-3776]
dc.identifier.orcidKoblar, S. [0000-0002-8667-203X]
dc.identifier.orcidKumar, S. [0000-0001-7126-9814]
dc.identifier.urihttp://hdl.handle.net/2440/59925
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.grantNHMRC
dc.rights© 2010 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
dc.source.urihttps://doi.org/10.1038/cdd.2010.12
dc.subjectNEDD1
dc.subjectcentrosome
dc.subjectzebrafish
dc.subjectdevelopment
dc.titleAn essential function for the centrosomal protein NEDD1 in zebrafish development
dc.typeJournal article
pubs.publication-statusPublished

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