Efficient microfluidic negative enrichment of circulating tumor cells in blood using roughened PDMS
Date
2015
Authors
Diéguez, L.M.
Winter, M.A.
Pocock, K.J.
Bremmell, K.E.
Thierry, B.
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Type:
Journal article
Citation
The Analyst, 2015; 140(10):3365-3372
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Abstract
Efficient isolation strategies not based on epithelial biomarker expression are required to enable nonbiased enrichment of circulating tumor cells (CTCs). CTCs undergoing epithelial-mesenchymal transition(EMT) may be prognostically relevant, and importantly are not detected with conventional epithelial based approaches such as Cell Search®. A method for the non-biased isolation of cancer cells within a peripheral blood sample utilizing microfluidic mixing PDMS devices functionalized with anti-CD45 is reported.The introduction of micro and nanoscale roughness using a single step treatment with sulfuric acid significantly increases the binding yield of white blood cells (WBCs) to the anti-CD45 conjugated surfaces.Up to 99.99% WBC depletion is achieved with a tumor cell recovery yield of 50%. This high level of CTC enrichment is expected to facilitate the detailed characterization of CTCs using for instance, imaging flow cytometry as demonstrated here.
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Dissertation Note
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Link to a related website: http://pdfs.semanticscholar.org/7b5e/a2258040d61cae85c0c25b9fffa1bc485844.pdf, Open Access via Unpaywall
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Copyright 2015 The Royal Society of Chemistry