CRLF2-positive B-cell acute lymphoblastic leukemia in adult patients : a single-institution experience

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dc.contributor.authorKonoplev, S.
dc.contributor.authorLu, X.
dc.contributor.authorKonopleva, M.
dc.contributor.authorJain, N.
dc.contributor.authorOuyang, J.
dc.contributor.authorGoswami, M.
dc.contributor.authorRoberts, K.
dc.contributor.authorValentine, M.
dc.contributor.authorMullighan, C.
dc.contributor.authorBueso-Ramos, C.
dc.contributor.authorZweidler-McKay, P.
dc.contributor.authorJorgensen, J.
dc.contributor.authorWang, S.
dc.date.issued2017
dc.description.abstractCytokine receptor-like factor 2 ( CRLF2 ) rearrangement is found in approximately 50% of pediatric Ph-like B-cell acute lymphoblastic leukemia (B-ALL), and around 50% of CRLF2 + cases harbor JAK mutations. We analyzed CRLF2 expression and studied its correlation with CRLF2 rearrangement in adult patients with B-ALL.Multiparameter flow cytometry (MFC) was performed consecutively in 126 patients.CRLF2 overexpression was detected in 30 (27%) patients, 28 (41%) of 69 patients with B-ALL not otherwise specified, 14 (21%) of 67 untreated patients, and 16 (27%) of 59 patients with relapsed B-ALL, with the highest among Hispanic patients (25/55, 45%). Of CRLF2+ cases, 21 (100%) of 21 cases showed CRLF2 rearrangement by fluorescence in situ hybridization, preferentially involving IGH@CRLF2 (15/15). The entire coding region of JAK2 was sequenced in 14 patients with CRLF2+ B-ALL, and nine (64%) were positive for JAK2 mutations.MFC allows a rapid, inexpensive, and reliable detection of B-ALL with CRLF2 rearrangement that would further facilitate testing for JAK2 mutations for targetable therapy.
dc.description.statementofresponsibilitySergej Konoplev, Xinyan Lu, Marina Konopleva, Nitin Jain, Juan Ouyang … Charles G. Mullighan … et al.
dc.identifier.citationAmerican Journal of Clinical Pathology, 2017; 147(4):357-363
dc.identifier.doi10.1093/AJCP/AQX005
dc.identifier.issn0002-9173
dc.identifier.issn1943-7722
dc.identifier.orcidMullighan, C. [0000-0002-1871-1850]
dc.identifier.urihttp://hdl.handle.net/2440/113046
dc.language.isoen
dc.publisherAmerican Society for Clinical Pathology
dc.rights© American Society for Clinical Pathology, 2017. All rights reserved.
dc.source.urihttps://doi.org/10.1093/ajcp/aqx005
dc.subjectB-lymphoblastic leukemia/lymphoma; BCR-ABL1–like; Ph-like B-ALL; CRLF2; Flow cytometry; FISH; JAK2 mutation
dc.titleCRLF2-positive B-cell acute lymphoblastic leukemia in adult patients : a single-institution experience
dc.typeJournal article
pubs.publication-statusPublished

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