The pulmonary surfactant system matures upon pipping in the freshwater turtle Chelydra serpentina

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dc.contributor.authorJohnston, Sonya D.en
dc.contributor.authorDaniels, Christopher Brianen
dc.contributor.authorCenzato, Daviden
dc.contributor.authorWhitsett, Jeffrey A.en
dc.contributor.authorOrgeig, Sandraen
dc.date.issued2002en
dc.description© The Company of Biologistsen
dc.description.abstractPulmonary surfactant (PS), a mixture of phospholipids (PL), neutral lipids and surfactant proteins (SP), lowers surface tension within the lung, which increases lung compliance and improves the removal of fluid at birth. Here, we have examined the expression of thyroid transcription factor-1 (TTF-1) and the surfactant protein SP-B, and also the composition of pulmonary surfactant lipids in the developing lung of the turtle Chelydra serpentina. Lavage and lung tissue were collected from late embryonic, pipped and hatchling turtles. TTF-1, a regulator of gene expression of surfactant proteins and cell differentiation in mammals, was detected using immunohistochemistry in epithelia of the gas-exchange area and conducting airways during late development. Expression declined in hatchlings. SP-B was detected in subsets of cells within the respiratory epithelium at all stages sampled. The same cell types also stained for TTF-1. Turtle surfactant lipids matured toward the end of incubation. Maximal secretion of both total phospholipids and disaturated phospholipid (DSP) occurred at the time of pipping, coincident with the onset of breathing. The DSP/PL ratio increased after pipping, whereas cholesterol levels (Chol) increased prior to pipping. This resulted in a decrease in the Chol/PL and Chol/DSP ratios after pipping. Thus, TTF-1 and SP-B appear to be highly conserved within the vertebrates. Maturation of surfactant phospholipid content occurred with the commencement of pulmonary ventilation.en
dc.description.statementofresponsibilitySonya D. Johnston, Christopher B. Daniels, David Cenzato, Jeffrey A. Whitsett and Sandra Orgeigen
dc.identifier.citationJournal of Experimental Biology, 2002; 205:415-425en
dc.identifier.issn0022-0949en
dc.identifier.urihttp://hdl.handle.net/2440/12190
dc.language.isoenen
dc.publisherCompany of Biologists Ltden
dc.source.urihttp://jeb.biologists.org/cgi/content/abstract/205/3/415en
dc.subjectThyroid transcription factor-1; surfactant protein; SP-B; phospholipid; cholesterol; lung; development; freshwater turtle; Chelydra serpentinaen
dc.titleThe pulmonary surfactant system matures upon pipping in the freshwater turtle Chelydra serpentinaen
dc.typeJournal articleen

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