Using an improved phagocytosis assay to evaluate the effect of HIV on specific antibodies to pregnancy-associated malaria

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dc.contributor.authorAtaide, R.
dc.contributor.authorHasang, W.
dc.contributor.authorWilson, D.
dc.contributor.authorBeeson, J.
dc.contributor.authorMwapasa, V.
dc.contributor.authorMolyneux, M.
dc.contributor.authorMeshnick, S.
dc.contributor.authorRogerson, S.
dc.contributor.editorSnounou, G.
dc.date.issued2010
dc.description.abstractBackground: Pregnant women residing in malaria endemic areas are highly susceptible to Plasmodium falciparum malaria, particularly during their first pregnancy, resulting in low birth weight babies and maternal anaemia. This susceptibility is associated with placental sequestration of parasitised red blood cells expressing pregnancy-specific variant surface antigens. Acquisition of antibodies against these variant surface antigens may protect women and their offspring. Functions of such antibodies may include prevention of placental sequestration or opsonisation of parasitised cells for phagocytic clearance. Methodology/Findings: Here we report the development and optimisation of a new high-throughput flow cytometry-based phagocytosis assay using undifferentiated Thp-1 cells to quantitate the amount of opsonizing antibody in patient sera, and apply this assay to measure the impact of HIV on the levels of antibodies to a pregnancy malaria-associated parasite line in a cohort of Malawian primigravid women. The assay showed high reproducibility, with inter-experimental correlation of r2 = 0.99. In primigravid women, concurrent malaria infection was associated with significantly increased antibodies, whereas HIV decreased the ability to acquire opsonising antibodies (Mann-Whitney ranksum: p = 0.013). This decrease was correlated with HIV-induced immunosuppression, with women with less than 350×106 CD4+ T- cells/L having less opsonising antibodies (coef: −11.95,P = 0.002). Levels of antibodies were not associated with protection from low birth weight or anaemia. Conclusions/Significance: This flow cytometry-based phagocytosis assay proved to be efficient and accurate for the measurement of Fc-receptor mediated phagocytosis-inducing antibodies in large cohorts. HIV was found to affect mainly the acquisition of antibodies to pregnancy-specific malaria in primigravidae. Further studies of the relationship between opsonising antibodies to malaria in pregnancy and HIV are indicated.
dc.description.statementofresponsibilityRicardo Ataíde, Wina Hasang, Danny W. Wilson, James G. Beeson, Victor Mwapasa, Malcolm E. Molyneux, Steven R. Meshnick, Stephen J. Rogerson
dc.identifier.citationPLoS One, 2010; 5(5):e10807-1-e10807-10
dc.identifier.doi10.1371/journal.pone.0010807
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.orcidWilson, D. [0000-0002-5073-1405]
dc.identifier.urihttp://hdl.handle.net/2440/83556
dc.language.isoen
dc.publisherPublic Library of Science
dc.relation.isreplacedby2440/89935
dc.relation.isreplacedbyhttp://hdl.handle.net/2440/89935
dc.rights© 2010 Ataíde et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.source.urihttps://doi.org/10.1371/journal.pone.0010807
dc.subjectCell Line
dc.subjectHumans
dc.subjectHIV
dc.subjectHIV Infections
dc.subjectPregnancy Complications, Parasitic
dc.subjectMalaria, Falciparum
dc.subjectAnemia
dc.subjectHemoglobins
dc.subjectImmunoglobulin G
dc.subjectAntibodies, Protozoan
dc.subjectAntigens, Protozoan
dc.subjectImmunoassay
dc.subjectCohort Studies
dc.subjectCell Differentiation
dc.subjectPhagocytosis
dc.subjectAntibody Specificity
dc.subjectPregnancy
dc.subjectInfant, Newborn
dc.subjectInfant, Low Birth Weight
dc.subjectFemale
dc.titleUsing an improved phagocytosis assay to evaluate the effect of HIV on specific antibodies to pregnancy-associated malaria
dc.typeJournal article
pubs.publication-statusPublished

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