Muscle stem cells undergo extensive clonal drift during tissue growth via meox1-mediated induction of G2 cell-cycle arrest
Date
2017
Authors
Nguyen, P.D.
Gurevich, D.B.
Sonntag, C.
Hersey, L.
Alaei, S.
Nim, H.T.
Siegel, A.
Hall, T.E.
Rossello, F.J.
Boyd, S.E.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Cell Stem Cell, 2017; 21(1):107-119
Statement of Responsibility
Phong Dang Nguyen, David Baruch Gurevich, Carmen Sonntag, Lucy Hersey, Sara Alaei, Hieu Tri Nim ... al et.
Conference Name
Abstract
Organ growth requires a careful balance between stem cell self-renewal and lineage commitment to ensure proper tissue expansion. The cellular and molecular mechanisms that mediate this balance are unresolved in most organs, including skeletal muscle. Here we identify a long-lived stem cell pool that mediates growth of the zebrafish myotome. This population exhibits extensive clonal drift, shifting from random deployment of stem cells during development to reliance on a small number of dominant clones to fuel the vast majority of muscle growth. This clonal drift requires Meox1, a homeobox protein that directly inhibits the cell-cycle checkpoint gene ccnb1. Meox1 initiates G<sub>2</sub> cell-cycle arrest within muscle stem cells, and disrupting this G<sub>2</sub> arrest causes premature lineage commitment and the resulting defects in muscle growth. These findings reveal that distinct regulatory mechanisms orchestrate stem cell dynamics during organ growth, beyond the G<sub>0</sub>/G<sub>1</sub> cell-cycle inhibition traditionally associated with maintaining tissue-resident stem cells.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© 2017 Published by Elsevier Inc.