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Expression of the costimulator molecules, CD40 and CD154, on lymphocytes from neonates and young children

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dc.contributor.authorElliott, S.
dc.contributor.authorRoberton, D.
dc.contributor.authorZola, H.
dc.contributor.authorMacardle, P.
dc.date.issued2000
dc.description.abstractDifferential expression of the costimulator molecules CD40 and CD154 on neonatal lymphocytes may be one explanation for limited T-dependent antibody responses in human neonates. CD40 was expressed at similar levels on resting B cells from adults, young children (2-20 months of age) or cord blood. CD40 expression was higher on cord blood B cells compared to adult B cells after stimulation with PMA and ionomycin, but similar on adult and cord blood B cells activated by CD3-stimulated T cells. In contrast to previous reports, cord blood T cells stimulated with PMA and ionomycin expressed adult levels of CD154 initially, but this expression was more transient on cord blood T cells. When adult and cord blood mononuclear cells were stimulated with CD3 mAb, T cells from some cord blood specimens showed different kinetics of CD154 expression compared with adult T cells. However, some cord blood specimens showed adult patterns of T cell CD154 expression. When mononuclear cells were depleted of B cells and monocytes prior to stimulation with CD3 mAb, the MFI and percentage of T cells expressing CD154 increased, with adult and cord T cells showing similar patterns of expression. These results show some differences in expression of CD40 and CD154 between neonatal and adult lymphocytes, but do not directly account for the relative deficiencies of humoral immunity in neonates.
dc.identifier.citationHuman Immunology, 2000; 61(4):378-388
dc.identifier.doi10.1016/S0198-8859(99)00189-5
dc.identifier.issn0198-8859
dc.identifier.issn1879-1166
dc.identifier.urihttp://hdl.handle.net/2440/7336
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.grantNHMRC
dc.source.urihttps://doi.org/10.1016/s0198-8859(99)00189-5
dc.subjectB-Lymphocyte Subsets
dc.subjectT-Lymphocyte Subsets
dc.subjectFetal Blood
dc.subjectHumans
dc.subjectMembrane Glycoproteins
dc.subjectCD40 Ligand
dc.subjectAntibodies, Monoclonal
dc.subjectLymphocyte Activation
dc.subjectInterphase
dc.subjectTime Factors
dc.subjectAdult
dc.subjectInfant
dc.subjectInfant, Newborn
dc.subjectCD3 Complex
dc.subjectLeukocyte Common Antigens
dc.subjectCD40 Antigens
dc.titleExpression of the costimulator molecules, CD40 and CD154, on lymphocytes from neonates and young children
dc.typeJournal article
pubs.publication-statusPublished

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