Terminal deoxynucleotidyl transferase (TdT)-mediated dutp nick-end labeling (TUNEL) for detection of apoptotic cells in Drosophila
Date
2015
Authors
Denton, D.
Kumar, S.
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Journal article
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Cold Spring Harbor Protocols, 2015; 2015(6):568-571
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Abstract
A characteristic feature of apoptosis is DNA fragmentation. This fragmentation can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) of DNA in dying cells. Here, we present a protocol for TUNEL detection of apoptosis in Drosophila larval tissue, but these techniques can be adapted for other tissues and developmental stages.
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Copyright 2015 Cold Spring Harbor Laboratory Press