Intergenomic SNPs reveal putative spontaneous chromosomal interchanges between chromosomes 7A and 7D of wheat

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dc.contributor.advisorMather, Diane Elizabeth
dc.contributor.authorNguyen, Phuoc Xuan
dc.contributor.schoolSchool of Agriculture, Food and Wineen
dc.date.issued2016
dc.descriptionTitle page, abstract and table of contents only. The complete thesis in print form is available from the University of Adelaide Library.
dc.description.abstractLangdon 7D(7A) and 7D(7B) durum substitution lines were crossed with DBA-Aurora durum wheat to introgress a lutein esterification gene,TaGelp1, from chromosome 7D onto its homoeologues 7A and 7B. Genotyping-by-sequencing based on DNA samples from durum wheat and bread wheat revealed single nucleotide polymorphism (SNPs) among the group-7 chromosomes. Sixteen KASP markers were developed and to be able to differentiate among these chromosomes. Nine 7A-7D markers were used to characterise progeny populations to search for dissociation of molecular markers which may indicate chromosomal recombination. Evidence of possible 7A-7D recombination was found in a small number of progeny (less than 4%). Most of the putative marker dissociations were in the centromeric region but one plant was found to carry only a small distal fragment of 7DS including TaGelp1. The findings suggest crossing normal durum with Langdon 7D(7A) combined with KASP marker assistance can be applied as a method to introgress and assess genes from chromosome 7D onto its homoeologues without resorting to use of wheat with the Ph1 deletion.en
dc.description.dissertationThesis (M.Bio.(PB)) -- University of Adelaide, Masters of Biotechnology (Plant Biotechnology), School of Agriculture, Food and Wine, 2016.en
dc.identifier.urihttp://hdl.handle.net/2440/112722
dc.provenanceMasters of Biotechnology (Plant Biotechnology) by courseworken
dc.subjectcourseworken
dc.subjectTriticum turgidum subsp. durumen
dc.subjectlutein esterificationen
dc.subjectgenotyping-by-sequencingen
dc.subjectKASP markeren
dc.subjecthomoeologous recombinationen
dc.titleIntergenomic SNPs reveal putative spontaneous chromosomal interchanges between chromosomes 7A and 7D of wheaten
dc.typeThesesen

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