Rat ovary produces cytokines during ovulation

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dc.contributor.authorBrannstrom, M.
dc.contributor.authorNorman, R.J.
dc.contributor.authorSeamark, R.F.
dc.contributor.authorRobertson, S.A.
dc.date.issued1994
dc.description.abstractTo examine the production of cytokines by the ovary during ovulation, ovaries were obtained from immature rats and from eCG/hCG-primed immature rats at different stages of the ovulatory process (before hCG injection, 10 h after hCG, and 20 h after hCG) and were perfused in vitro for 5 h. Large quantities of interleukin (IL)-6 and granulocyte-macrophage colony-stimulating factor (GM-CSF) bioactivity and smaller amounts of tumor necrosis factor-alpha (TNF alpha) and IL-1 bioactivity were found in the perfusate. IL-2 and IL-3 were not detectable in the perfusion media. The GM-CSF content was significantly higher in the perfusate of ovulating ovaries (obtained 10 h after hCG) compared to the earlier stages. Studies on preovulatory ovaries (prior to hCG injection) revealed that GM-CSF release was not influenced by LH, but was markedly increased when recombinant human IL-1 beta (4 ng/ml) was added to the perfusion medium. IL-6 was released in similar amounts from ovaries at all stages. The identity of bioactive GM-CSF was confirmed by neutralization with a specific polyclonal antibody against murine GM-CSF. Size-exclusion chromatography of perfusion medium revealed peaks of GM-CSF and IL-6 bioactivity at approximate molecular masses of 21-23 kDa and 24-25 kDa, respectively. This study demonstrates that the rat ovary produces IL-6, GM-CSF, TNF alpha, and IL-1 prior to and during the ovulatory process and that there are temporal fluctuations in GM-CSF release with a peak in output at ovulation.
dc.description.statementofresponsibilityMats Brännström, Robert J. Norman, Robert F. Seamark and Sarah A. Robertson
dc.identifier.citationBiology of Reproduction, 1994; 50(1):88-94
dc.identifier.doi10.1095/biolreprod50.1.88
dc.identifier.issn0006-3363
dc.identifier.issn1529-7268
dc.identifier.orcidNorman, R.J. [0000-0002-3118-3896]
dc.identifier.orcidRobertson, S.A. [0000-0002-9967-0084]
dc.identifier.urihttp://hdl.handle.net/2440/85265
dc.language.isoen
dc.publisherSociety for the Study of Reproduction
dc.rightsCopyright status unknown
dc.source.urihttps://doi.org/10.1095/biolreprod50.1.88
dc.subjectOvary
dc.subjectAnimals
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectEstradiol
dc.subjectProgesterone
dc.subjectChorionic Gonadotropin
dc.subjectTumor Necrosis Factor-alpha
dc.subjectGranulocyte-Macrophage Colony-Stimulating Factor
dc.subjectInterleukin-6
dc.subjectAntibodies
dc.subjectCytokines
dc.subjectOvulation
dc.subjectMolecular Weight
dc.subjectFemale
dc.titleRat ovary produces cytokines during ovulation
dc.typeJournal article
pubs.publication-statusPublished

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