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Regulation of osteopontin expression by type 1 collagen in preosteoblastic UMR201 cells

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dc.contributor.authorTraianedes, K.
dc.contributor.authorMartin, T.
dc.contributor.authorFindlay, D.
dc.date.issued1996
dc.description.abstractWhen UMR201 cells, phenotypically preosteoblastic, were placed onto a type I collagen gel, expression of osteopontin (OP) mRNA and protein were strongly upregulated, compared to cells plated onto plastic. This upregulation was dose-dependent, with respect to the concentration of collagen gel, and was observable within hours of cells having attached and spread on the substrate. Retinoic acid (RA) acted synergistically with type I collagen at each concentration to induce a much greater increase in OP mRNA than in cells on plastic. In addition, RA increased the phosphorylation of secreted OP. The exogenous collagen substrate inhibited the growth of UMR201 cells, with the extent and duration of inhibition dependent on the collagen concentration. The effect of type I collagen was specific; plating cells on fibronectin, laminin or vitronectin did not upregulate OP expression. In contrast to the effects on OP expression, the strong RA induction of alkaline phosphatase (ALP) mRNA in cells on plastic was attenuated in cells plated on type I collagen. Growth on type I collagen did not change OP mRNA stability or transcription rate, although there was decreased stability of the ALP mRNA in cells on collagen.
dc.identifier.citationConnective Tissue Research, 1996; 34(1):63-74
dc.identifier.doi10.3109/03008209609028894
dc.identifier.issn0300-8207
dc.identifier.issn1607-8438
dc.identifier.urihttp://hdl.handle.net/2440/6689
dc.language.isoen
dc.publisherInforma UK Limited
dc.source.urihttps://doi.org/10.3109/03008209609028894
dc.subjectCell Line
dc.subjectOsteoblasts
dc.subjectAnimals
dc.subjectHumans
dc.subjectRats
dc.subjectAscorbic Acid
dc.subjectTretinoin
dc.subjectCollagen
dc.subjectAlkaline Phosphatase
dc.subjectGlyceraldehyde-3-Phosphate Dehydrogenases
dc.subjectSialoglycoproteins
dc.subjectVitronectin
dc.subjectFibronectins
dc.subjectLaminin
dc.subjectReceptors, Retinoic Acid
dc.subjectCell Adhesion
dc.subjectCell Division
dc.subjectTranscription, Genetic
dc.subjectGene Expression Regulation
dc.subjectOsteopontin
dc.titleRegulation of osteopontin expression by type 1 collagen in preosteoblastic UMR201 cells
dc.typeJournal article
pubs.publication-statusPublished

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