An investigation of boron toxicity in barley using metabolomics

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dc.contributor.authorRoessner, U.
dc.contributor.authorPatterson, J.
dc.contributor.authorForbes, M.
dc.contributor.authorFincher, G.
dc.contributor.authorLangridge, P.
dc.contributor.authorBacic, A.
dc.date.issued2006
dc.description© 2006 American Society of Plant Biologists
dc.description.abstractBoron (B) is an essential micronutrient that affects plant growth at either deficient or toxic concentrations in soil. The aim of this work was to investigate the adaptation of barley (Hordeum vulgare) plants to toxic B levels and to increase our understanding of B toxicity tolerance mechanisms. We used a metabolomics approach to compare metabolite profiles in root and leaf tissues of an intolerant, commercial cultivar (cv Clipper) and a B-tolerant Algerian landrace (cv Sahara). After exposure to elevated B (200 and 1,000 µM), the number and amplitude of metabolite changes in roots was greater in Clipper than in Sahara. In contrast, leaf metabolites of both cultivars only responded following 1,000 µM treatment, at which B toxicity symptoms (necrosis) were visible. In addition, metabolite levels were dramatically altered in the tips of leaves of the sensitive cultivar Clipper after growth in 1,000 µM B compared to those of Sahara. This correlates with a gradual accumulation of B from leaf base to tip in B-intolerant cultivars. Overall, there were always greater differences between tissue types (roots and leaves) than between the two cultivars. This work has provided insights into metabolic differences of two genetically distinct barley cultivars and information about how they respond metabolically to increasing B levels.
dc.description.statementofresponsibilityUte Roessner, John H. Patterson, Megan G. Forbes, Geoffrey B. Fincher, Peter Langridge, and Anthony Bacic
dc.identifier.citationPlant Physiology, 2006; 142(3):1087-1101
dc.identifier.doi10.1104/pp.106.084053
dc.identifier.issn0032-0889
dc.identifier.issn1532-2548
dc.identifier.orcidLangridge, P. [0000-0001-9494-400X]
dc.identifier.urihttp://hdl.handle.net/2440/23967
dc.language.isoen
dc.publisherAmer Soc Plant Physiologists
dc.source.urihttps://doi.org/10.1104/pp.106.084053
dc.subjectPolysaccharide rhamnogalacturonan-ii
dc.subjectplant-cell-walls
dc.subjectpectic polysaccharide
dc.subjectdrought stress
dc.subjectmetabolites
dc.subjecttolerance
dc.subjectidentification
dc.subjectmechanism
dc.subjectreveals
dc.subjectcomplex
dc.titleAn investigation of boron toxicity in barley using metabolomics
dc.typeJournal article
pubs.publication-statusPublished

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