Current studentsExisting staffContact us
 

Neural crest cell-derived VEGF promotes embryonic jaw extension

Simple item page
dc.contributor.authorWiszniak, S.
dc.contributor.authorMackenzie, F.E.
dc.contributor.authorAnderson, P.
dc.contributor.authorKabbara, S.
dc.contributor.authorRuhrberg, C.
dc.contributor.authorSchwarz, Q.
dc.date.issued2015
dc.description.abstractJaw morphogenesis depends on the growth of Meckel's cartilage during embryogenesis. However, the cell types and signals that promote chondrocyte proliferation for Meckel's cartilage growth are poorly defined. Here we show that neural crest cells (NCCs) and their derivatives provide an essential source of the vascular endothelial growth factor (VEGF) to enhance jaw vascularization and stabilize the major mandibular artery. We further show in two independent mouse models that blood vessels promote Meckel's cartilage extension. Coculture experiments of arterial tissue with NCCs or chondrocytes demonstrated that NCC-derived VEGF promotes blood vessel growth and that blood vessels secrete factors to instruct chondrocyte proliferation. Computed tomography and X-ray scans of patients with hemifacial microsomia also showed that jaw hypoplasia correlates with mandibular artery dysgenesis. We conclude that cranial NCCs and their derivatives provide an essential source of VEGF to support blood vessel growth in the developing jaw, which in turn is essential for normal chondrocyte proliferation, and therefore jaw extension.
dc.description.statementofresponsibilitySophie Wiszniak, Francesca E. Mackenzie, Peter Anderson, Samuela Kabbara, Christiana Ruhrberg, and Quenten Schwarz
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 2015; 112(19):6086-6091
dc.identifier.doi10.1073/pnas.1419368112
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.orcidWiszniak, S. [0000-0001-8666-9300]
dc.identifier.orcidAnderson, P. [0000-0002-3730-4652]
dc.identifier.urihttps://hdl.handle.net/2440/134088
dc.language.isoen
dc.publisherNational Academy of Sciences
dc.relation.grantNHMRC
dc.rightsFreely available online through the PNAS open access option.
dc.source.urihttps://doi.org/10.1073/pnas.1419368112
dc.subjectNeural crest; VEGF; mandible; chondrocyte; blood vessel
dc.subject.meshCartilage
dc.subject.meshMandible
dc.subject.meshChondrocytes
dc.subject.meshNeural Crest
dc.subject.meshAnimals
dc.subject.meshHumans
dc.subject.meshMice
dc.subject.meshGoldenhar Syndrome
dc.subject.meshVascular Endothelial Growth Factor A
dc.subject.meshTomography, X-Ray Computed
dc.subject.meshCoculture Techniques
dc.subject.meshIn Situ Hybridization
dc.subject.meshCell Differentiation
dc.subject.meshCell Proliferation
dc.subject.meshAdolescent
dc.subject.meshFemale
dc.subject.meshMale
dc.subject.meshWnt1 Protein
dc.titleNeural crest cell-derived VEGF promotes embryonic jaw extension
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Medicine publications