Qualitative and quantitative event-specific PCR detection methods for Oxy-235 canola based on the 3' integration flanking sequence

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dc.contributor.authorYang, L.
dc.contributor.authorGuo, J.
dc.contributor.authorZhang, H.
dc.contributor.authorLiu, J.
dc.contributor.authorZhang, D.
dc.date.issued2008
dc.description.abstractAs more genetically modified plant events are approved for commercialization worldwide, the event-specific PCR method has become the key method for genetically modified organism (GMO) identification and quantification. This study reveals the 3' flanking sequence of the exogenous integration of Oxy-235 canola employing thermal asymmetric interlaced PCR (TAIL-PCR). On the basis of the revealed 3' flanking sequence, PCR primers and TaqMan probe were designed and qualitative and quantitative PCR assays were established for Oxy-235 canola. The specificity and limits of detection (LOD) and quantification (LOQ) of these two PCR assays were validated to as low as 0.1% for the relative LOD of qualitative PCR assay; the absolute LOD and LOQ were low to 10 and 20 copies of canola genomic DNA in quantitative PCR assay, respectively. Furthermore, ideal quantified results were obtained in the practical canola sample detection. All of the results indicate that the developed qualitative and quantitative PCR methods based on the revealed 3' integration flanking sequence are suitable for GM canola Oxy-235 identification and quantification.
dc.description.statementofresponsibilityLitao Yang, Jinchao Guo, Haibo Zhang, Jia Liu and Dabing Zhang
dc.identifier.citationJournal of Agricultural and Food Chemistry, 2008; 56(6):1804-1809
dc.identifier.doi10.1021/jf073465i
dc.identifier.issn0021-8561
dc.identifier.issn1520-5118
dc.identifier.orcidZhang, D. [0000-0003-3181-9812]
dc.identifier.urihttp://hdl.handle.net/2440/89802
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.rights© 2008 American Chemical Society
dc.source.urihttps://doi.org/10.1021/jf073465i
dc.subjectGenetically modified organisms; event-specific; Oxy-235 canola; TAIL-PCR; real-time PCR
dc.titleQualitative and quantitative event-specific PCR detection methods for Oxy-235 canola based on the 3' integration flanking sequence
dc.typeJournal article
pubs.publication-statusPublished

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