Transcriptome analysis of Thapsia laciniata rouy provides insights into terpenoid biosynthesis and diversity in apiaceae

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dc.contributor.authorDrew, D.
dc.contributor.authorDueholm, B.
dc.contributor.authorWeitzel, C.
dc.contributor.authorZhang, Y.
dc.contributor.authorSensen, C.
dc.contributor.authorSimonsen, H.
dc.date.issued2013
dc.description.abstractThapsia laciniata Rouy (Apiaceae) produces irregular and regular sesquiterpenoids with thapsane and guaiene carbon skeletons, as found in other Apiaceae species. A transcriptomic analysis utilizing Illumina next-generation sequencing enabled the identification of novel genes involved in the biosynthesis of terpenoids in Thapsia. From 66.78 million HQ paired-end reads obtained from T. laciniata roots, 64.58 million were assembled into 76,565 contigs (N50: 1261 bp). Seventeen contigs were annotated as terpene synthases and five of these were predicted to be sesquiterpene synthases. Of the 67 contigs annotated as cytochromes P450, 18 of these are part of the CYP71 clade that primarily performs hydroxylations of specialized metabolites. Three contigs annotated as aldehyde dehydrogenases grouped phylogenetically with the characterized ALDH1 from Artemisia annua and three contigs annotated as alcohol dehydrogenases grouped with the recently described ADH1 from A. annua. ALDH1 and ADH1 were characterized as part of the artemisinin biosynthesis. We have produced a comprehensive EST dataset for T. laciniata roots, which contains a large sample of the T. laciniata transcriptome. These transcriptome data provide the foundation for future research into the molecular basis for terpenoid biosynthesis in Thapsia and on the evolution of terpenoids in Apiaceae.
dc.description.statementofresponsibilityDamian Paul Drew, Bjørn Dueholm, Corinna Weitzel, Ye Zhang, Christoph W. Sensen and Henrik Toft Simonsen
dc.identifier.citationInternational Journal of Molecular Sciences, 2013; 14(5):9080-9098
dc.identifier.doi10.3390/ijms14059080
dc.identifier.issn1661-6596
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/2440/79105
dc.language.isoen
dc.publisherMDPI AG
dc.rights© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
dc.source.urihttps://doi.org/10.3390/ijms14059080
dc.subjectThapsia laciniata
dc.subjectguaianolides
dc.subjectthapsane
dc.subjectApiaceae
dc.subjectsesquiterpenoids
dc.titleTranscriptome analysis of Thapsia laciniata rouy provides insights into terpenoid biosynthesis and diversity in apiaceae
dc.typeJournal article
pubs.publication-statusPublished

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