Pentosan polysulfate promotes proliferation and chondrogenic differentiation of adult human bone marrow-derived mesenchymal precursor cells

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dc.contributor.authorGhosh, P.
dc.contributor.authorWu, J.
dc.contributor.authorShimmon, S.
dc.contributor.authorZannettino, A.
dc.contributor.authorGronthos, S.
dc.contributor.authorItescu, S.
dc.date.issued2010
dc.descriptionExtent: 17p.
dc.description.abstractIntroduction: This study was undertaken to determine whether the anti-osteoarthritis drug pentosan polysulfate (PPS) influenced mesenchymal precursor cell (MPC) proliferation and differentiation. Methods: Human MPCs were maintained in monolayer, pellet or micromass cultures (MMC) for up to 10 days with PPS at concentrations of 0 to 20 μg/ml. MPC viability and proliferation was assessed using the WST-1 assay and 3H-thymidine incorporation into DNA, while apoptosis was monitored by flow cytometry. Proteoglycan (PG) biosynthesis was determined by 35SO4 2- incorporation and staining with Alcian blue. Proteoglycan and collagen type I and collagen type II deposition in pellet cultures was also examined by Toluidine blue and immunohistochemical staining, respectively. The production of hyaluronan (HA) by MPCs in MMC was assessed by ELISA. The relative outcome of PPS, HA, heparin or dextran sulfate (DS) on PG synthesis was compared in 5-day MMC. Gene expression of MPCs in 7-day and 10-day MMC was examined using real-time PCR. MPC differentiation was investigated by co-culturing with PPS in osteogenic or adipogenic inductive culture media for 28 days. Results: Significant MPC proliferation was evident by day 3 at PPS concentrations of 1 to 5 μg/ml (P < 0.01). In the presence of 1 to 10 μg/ml PPS, a 38% reduction in IL-4/IFNγ-induced MPC apoptosis was observed. In 5-day MMC, 130% stimulation of PG synthesis occurred at 2.5 μg/ml PPS (P < 0.0001), while 5.0 μg/ml PPS achieved maximal stimulation in the 7-day and 10-day cultures (P < 0.05). HA and DS at ≥ 5 μg/ml inhibited PG synthesis (P < 0.05) in 5-day MMC. Collagen type II deposition by MMC was significant at ≥ 0.5 μg/ml PPS (P < 0.001 to 0.05). In MPC-PPS pellet cultures, more PG, collagen type II but less collagen type I was deposited than in controls. Real-time PCR results were consistent with the protein data. At 5 and 10 μg/ml PPS, MPC osteogenic differentiation was suppressed (P < 0.01). Conclusions: This is the first study to demonstrate that PPS promotes MPC proliferation and chondrogenesis, offering new strategies for cartilage regeneration and repair in osteoarthritic joints.
dc.description.statementofresponsibilityPeter Ghosh, Jiehua Wu, Susan Shimmon, Andrew CW Zannettino, Stan Gronthos and Silviu Itescu
dc.identifier.citationArthritis Research & Therapy, 2010; 12(1):R28-1-R28-17
dc.identifier.doi10.1186/ar2935
dc.identifier.issn1478-6362
dc.identifier.issn1478-6362
dc.identifier.orcidZannettino, A. [0000-0002-6646-6167]
dc.identifier.orcidGronthos, S. [0000-0002-6225-3084]
dc.identifier.urihttp://hdl.handle.net/2440/63028
dc.language.isoen
dc.publisherBioMed Central Ltd.
dc.rights© 2010 Ghosh et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttps://doi.org/10.1186/ar2935
dc.subjectBone Marrow Cells
dc.subjectChondrocytes
dc.subjectMesenchymal Stem Cells
dc.subjectHumans
dc.subjectCollagen
dc.subjectHyaluronic Acid
dc.subjectPentosan Sulfuric Polyester
dc.subjectProteoglycans
dc.subjectAnti-Inflammatory Agents
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFlow Cytometry
dc.subjectCell Separation
dc.subjectImmunohistochemistry
dc.subjectGene Expression Profiling
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectCell Differentiation
dc.subjectCell Proliferation
dc.subjectGene Expression
dc.subjectChondrogenesis
dc.subjectAdult
dc.titlePentosan polysulfate promotes proliferation and chondrogenic differentiation of adult human bone marrow-derived mesenchymal precursor cells
dc.typeJournal article
pubs.publication-statusPublished

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