Mechanistic insight in surface nanotopography driven cellular migration

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dc.contributor.authorDabare, P.R.L.
dc.contributor.authorBachhuka, A.
dc.contributor.authorVisalakshan, R.M.
dc.contributor.authorShirazi, H.S.
dc.contributor.authorOstriko, K.
dc.contributor.authorSmith, L.E.
dc.contributor.authorVasilev, K.
dc.date.issued2021
dc.description.abstractCellular migration plays a vital role in many physiological processes. To elucidate the role of surface nanotopography on the downstream signaling pathways underlying cell migration, model surfaces having well-defined hill-like surface nanotopography and uniform surface chemistry were designed and implemented using plasma polymerization and covalent attachment of nanoparticles of predetermined size. A scratch wound assay, immunostaining, and gene expression of focal adhesion (FA) proteins were performed to determine the influence of surface nanotopography on cell migration. The results of this study demonstrate that the gap closure between cell monolayers is faster on surfaces having greater nanoscale topography. The phenomenon is predominantly driven by cell migration and was independent from cell proliferation. Qualitative and quantitative assessment of proteins involved in the signaling pathways underlying cell migration showed significant modulation by surface nanotopography. Specifically, focal adhesion sites decreased with the increase in surface nanotopography scale while the expression of FA proteins increased. This implies that nanotopography mediated modulation of cell migration is directly governed by the recruitment of receptor and adapter proteins responsible for cell-surface interaction. The results of this study indicate that biomaterial devices and constructs having rationally designed surface nanotopography and chemistry could be utilized to regulate wound healing and tissue regeneration.
dc.description.statementofresponsibilityPanthihage Ruvini L. Dabare, Akash Bachhuka, Rahul M. Visalakshan, Hanieh S. Shirazi, Kostya Ostriko, Louise E. Smith, and Krasimir Vasilev
dc.identifier.citationACS Biomaterials Science and Engineering, 2021; 7(10):4921-4932
dc.identifier.doi10.1021/acsbiomaterials.1c00853
dc.identifier.issn2373-9878
dc.identifier.issn2373-9878
dc.identifier.orcidBachhuka, A. [0000-0003-1253-8126]
dc.identifier.urihttps://hdl.handle.net/2440/132911
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.granthttp://purl.org/au-research/grants/arc/DP180101254
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1194466
dc.rights© 2021 American Chemical Society
dc.source.urihttps://doi.org/10.1021/acsbiomaterials.1c00853
dc.subjectCell migration; nanotopography; plasma polymerization; surface chemistry; wound healing; biomaterials
dc.subject.meshCell Adhesion
dc.subject.meshCell Movement
dc.subject.meshCell Proliferation
dc.subject.meshFocal Adhesions
dc.subject.meshSurface Properties
dc.titleMechanistic insight in surface nanotopography driven cellular migration
dc.typeJournal article
pubs.publication-statusPublished

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