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Isolation, propagation and characterization of cord blood derived CD4+ CD25+ regulatory T cells

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dc.contributor.authorBresatz, S.
dc.contributor.authorSadlon, T.
dc.contributor.authorMillard, D.
dc.contributor.authorZola, H.
dc.contributor.authorBarry, S.
dc.date.issued2007
dc.descriptionCopyright © 2007 Elsevier B.V. All rights reserved.
dc.description.abstractRegulatory T cells (Treg) have recently come to the fore in studies of immune regulation, particularly in autoimmune disease and cancer. While there appear to be several distinct subsets of T cells with regulatory function, a population described as natural Treg and characterized by expression of the transcription factor FOXP3 has attracted particular interest. These cells can be enriched using the surface markers CD4 and CD25, and cord blood is a convenient source of CD25+ Treg. We present detailed protocols for the enrichment of Treg from cord blood using CD25 and a magnetic bead procedure, yielding populations >80% positive for CD25 and 50-65% FOXP3 positive. This enrichment can be followed by a second magnetic bead or a flow sorting step, yielding >95% CD25 and >65% FOXP3 positive populations. Protocols are presented for propagation of these cells in culture (yielding >80% FOXP3 positive cells) and for their phenotypic and functional characterization.
dc.description.statementofresponsibilitySuzanne Bresatz, Tim Sadlon, Debrah Millard, Heddy Zola and Simon C. Barry
dc.description.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/506022/description#description
dc.identifier.citationJournal of Immunological Methods, 2007; 327(1-2):53-62
dc.identifier.doi10.1016/j.jim.2007.06.006
dc.identifier.issn0022-1759
dc.identifier.issn1872-7905
dc.identifier.orcidSadlon, T. [0000-0002-4821-2462]
dc.identifier.orcidBarry, S. [0000-0002-0597-7609]
dc.identifier.urihttp://hdl.handle.net/2440/44025
dc.language.isoen
dc.publisherElsevier Science BV
dc.source.urihttps://doi.org/10.1016/j.jim.2007.06.006
dc.subjectT-Lymphocyte Subsets
dc.subjectFetal Blood
dc.subjectHumans
dc.subjectFlow Cytometry
dc.subjectCell Culture Techniques
dc.subjectImmunomagnetic Separation
dc.subjectPregnancy
dc.subjectFemale
dc.subjectT-Lymphocytes, Regulatory
dc.subjectInterleukin-2 Receptor alpha Subunit
dc.subjectCD4 Antigens
dc.titleIsolation, propagation and characterization of cord blood derived CD4+ CD25+ regulatory T cells
dc.typeJournal article
pubs.publication-statusPublished

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