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Proteomic responses to gold(III)-toxicity in the bacterium Cupriavidus metallidurans CH34

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dc.contributor.authorZammit, C.
dc.contributor.authorWeiland, F.
dc.contributor.authorBrugger, J.
dc.contributor.authorWade, B.
dc.contributor.authorWinderbaum, L.
dc.contributor.authorNies, D.
dc.contributor.authorSoutham, G.
dc.contributor.authorHoffmann, P.
dc.contributor.authorReith, F.
dc.date.issued2016
dc.descriptionAccepted 11th October 2016 Link to a related website: https://digital.library.adelaide.edu.au/dspace/bitstream/2440/102854/2/hdl_102854.pdf, Open Access via Unpaywall
dc.description.abstractThe metal-resistant β-proteobacterium Cupriavidus metallidurans drives gold (Au) biomineralisation and the (trans)formation of Au nuggets largely via unknown biochemical processes, ultimately leading to the reductive precipitation of mobile, toxic Au(i/iii)-complexes. In this study proteomic responses of C. metallidurans CH34 to mobile, toxic Au(iii)-chloride are investigated. Cells were grown in the presence of 10 and 50 μM Au(iii)-chloride, 50 μM Cu(ii)-chloride and without additional metals. Differentially expressed proteins were detected by difference gel electrophoresis and identified by liquid chromatography coupled mass spectrometry. Proteins that were more abundant in the presence of Au(iii)-chloride are involved in a range of important cellular functions, e.g., metabolic activities, transcriptional regulation, efflux and metal transport. To identify Au-binding proteins, protein extracts were separated by native 2D gel electrophoresis and Au in protein spots was detected by laser absorption inductively coupled plasma mass spectrometry. A chaperon protein commonly understood to bind copper (Cu), CupC, was identified and shown to bind Au. This indicates that it forms part of a multi-metal detoxification system and suggests that similar/shared detoxification pathways for Au and Cu exist. Overall, this means that C. metallidurans CH34 is able to mollify the toxic effects of cytoplasmic Au(iii) by sequestering this Au-species. This effect may in the future be used to develop CupC-based biosensing capabilities for the in-field detection of Au in exploration samples.
dc.description.statementofresponsibilityCarla M. Zammit, Florian Weiland, Joël Brugger, Benjamin Wade, Lyron Juan Winderbaum, Dietrich H. Nies, Gordon Southam, Peter Hoffmann and Frank Reith
dc.identifier.citationMetallomics, 2016; 8(11):1204-1216
dc.identifier.doi10.1039/c6mt00142d
dc.identifier.issn1756-5901
dc.identifier.issn1756-591X
dc.identifier.orcidWeiland, F. [0000-0001-9702-9245]
dc.identifier.orcidHoffmann, P. [0000-0002-6573-983X]
dc.identifier.urihttp://hdl.handle.net/2440/102854
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.granthttp://purl.org/au-research/grants/arc/LP100200102
dc.relation.granthttp://purl.org/au-research/grants/arc/FT150100250
dc.rightsThis journal is © The Royal Society of Chemistry 2016
dc.source.urihttp://dx.doi.org/10.1039/c6mt00142d
dc.subjectswiss-model
dc.subjectcopper resistance
dc.subjectescherichia-coli
dc.subjectprotein
dc.subjectdehydrogenase
dc.subjectbiomineralization
dc.titleProteomic responses to gold(III)-toxicity in the bacterium Cupriavidus metallidurans CH34
dc.typeJournal article
pubs.publication-statusPublished

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