Dietary enrichment with fish oil prevents high fat-induced metabolic dysfunction in skeletal muscle in mice

Simple item page
dc.contributor.authorPhilp, L.
dc.contributor.authorHeilbronn, L.
dc.contributor.authorJanovska, A.
dc.contributor.authorWittert, G.
dc.contributor.editorAlemany, M.
dc.date.issued2015
dc.description.abstractHigh saturated fat (HF-S) diets increase intramyocellular lipid, an effect ameliorated by omega-3 fatty acids in vitro and in vivo, though little is known about sex- and muscle fiber type-specific effects. We compared effects of standard chow, HF-S, and 7.5% HF-S replaced with fish oil (HF-FO) diets on the metabolic profile and lipid metabolism gene and protein content in red (soleus) and white (extensor digitorum longus) muscles of male and female C57BL/6 mice (n = 9-12/group). Weight gain was similar in HF-S- and HF-FO-fed groups. HF-S feeding increased mesenteric fat mass and lipid marker, Oil Red O, in red and mixed muscle; HF-FO increased interscapular brown fat mass. Compared to chow, HF-S and HF-FO increased expression of genes regulating triacylglycerol synthesis and fatty acid transport, HF-S suppressed genes and proteins regulating fatty acid oxidation, whereas HF-FO increased oxidative genes, proteins and enzymes and lipolytic gene content, whilst suppressing lipogenic genes. In comparison to HF-S, HF-FO further increased fat transporters, markers of fatty acid oxidation and mitochondrial content, and reduced lipogenic genes. No diet-by-sex interactions were observed. Neither diet influenced fiber type composition. However, some interactions between muscle type and diet were observed. HF-S induced changes in triacylglycerol synthesis and lipogenic genes in red, but not white, muscle, and mitochondrial biogenesis and oxidative genes were suppressed by HF-S and increased by HF-FO in red muscle only. In conclusion, HF-S feeding promotes lipid storage in red muscle, an effect abrogated by the fish oil, which increases mediators of lipolysis, oxidation and thermogenesis while inhibiting lipogenic genes. Greater storage and synthesis, and lower oxidative genes in red, but not white, muscle likely contribute to lipid accretion encountered in red muscle. Despite several gender-dimorphic genes, both sexes exhibited a similar HF-S-induced metabolic and gene expression profile; likewise fish oil was similarly protective in both sexes.
dc.description.statementofresponsibilityLisa K. Philp, Leonie K. Heilbronn, Alena Janovska, Gary A. Wittert
dc.identifier.citationPLoS ONE, 2015; 10(2):e0117494-1-e0117494-19
dc.identifier.doi10.1371/journal.pone.0117494
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.orcidHeilbronn, L. [0000-0003-2106-7303]
dc.identifier.orcidWittert, G. [0000-0001-6818-6065]
dc.identifier.urihttp://hdl.handle.net/2440/90177
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights© 2015 Philp et al. This is an open access article distributed under the terms of the Creative Commons Attribution License [http://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.source.urihttps://doi.org/10.1371/journal.pone.0117494
dc.subjectMuscle, Skeletal
dc.subjectMitochondria
dc.subjectAnimals
dc.subjectMice, Inbred C57BL
dc.subjectMice
dc.subjectCarbon-Carbon Double Bond Isomerases
dc.subjectRacemases and Epimerases
dc.subjectEnoyl-CoA Hydratase
dc.subject3-Hydroxyacyl CoA Dehydrogenases
dc.subjectAcetyl-CoA C-Acyltransferase
dc.subjectTriglycerides
dc.subjectFish Oils
dc.subjectRNA, Messenger
dc.subjectOxidative Phosphorylation
dc.subjectFemale
dc.subjectMale
dc.subjectLipogenesis
dc.subjectLipid Metabolism
dc.subjectFatty Acid Transport Proteins
dc.subjectAdipose Tissue, Brown
dc.subjectAdipose Tissue, White
dc.subjectDiet, High-Fat
dc.titleDietary enrichment with fish oil prevents high fat-induced metabolic dysfunction in skeletal muscle in mice
dc.typeJournal article
pubs.publication-statusPublished

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
hdl_90177.pdf
Size:
2.03 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Medicine publications