Effect of specific phosphodiesterase isoenzyne inhibitors during in vitro maturation of bovine oocytes on meiotic and developmental capacity

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dc.contributor.authorThomas, R.
dc.contributor.authorThompson, J.
dc.contributor.authorArmstrong, D.
dc.contributor.authorGilchrist, R.
dc.date.issued2004
dc.description© 2004 by the Society for the Study of Reproduction, Inc.
dc.description.abstractCompared with oocytes matured in vivo, in vitro-matured oocytes are compromised in their capacity to support early embryo development. Delaying spontaneous in vitro meiotic maturation using specific phosphodiesterase (PDE) isoenzyme inhibitors may permit more complete oocyte cytoplasmic maturation, possibly by prolonging cumulus cell (CC)-oocyte gap junctional communication during meiotic resumption. This study aimed to investigate the effect of the isoenzyme 3- (oocyte) and isoenzyme 4- (granulosa cell) specific PDE inhibitors on the kinetics of in vitro maturation and on subsequent oocyte developmental competence. Cumulus-oocyte complexes from antral bovine follicles were isolated and cultured in the presence of the specific PDE inhibitors milrinone (type 3) or rolipram (type 4) (100 µM). In the presence of FSH, both PDE inhibitors only slightly extended CC-oocyte gap junctional communication over the first 9 h, but they completely blocked meiotic resumption during this period (P < 0.001). The indefinite inhibitory effect of milrinone on meiotic resumption (30% at germinal vesicle stage after 48 h) was overridden by 24 h when treated with FSH, but not with hCG, suggesting a form of induced meiotic resumption. Oocytes treated with FSH with or without either PDE inhibitor were inseminated at either 24, 26, or 28 h. Treated with either the type 3 or type 4 PDE inhibitor significantly (P < 0.05) increased embryo development to the blastocyst stage by 33%–39% (to an average of 52% blastocysts) compared with control oocytes (38%) after insemination at 28 h, and significantly (P < 0.05) increased blastocyst cell numbers when inseminated at 24 h. These results suggest that delayed spontaneous meiotic maturation, coupled with extended gap junctional communication between the CCs and the oocyte has a positive effect on oocyte cytoplasmic maturation, thereby improving oocyte developmental potential.
dc.description.statementofresponsibilityR.E. Thomas, J.G. Thompson, D.T. Armstrong, and R.B. Gilchrist
dc.identifier.citationBiology of Reproduction, 2004; 71(4):1142-1149
dc.identifier.doi10.1095/biolreprod.103.024828
dc.identifier.issn0006-3363
dc.identifier.issn1529-7268
dc.identifier.orcidThompson, J. [0000-0003-4941-7731]
dc.identifier.orcidGilchrist, R. [0000-0003-1611-7142]
dc.identifier.urihttp://hdl.handle.net/2440/8033
dc.language.isoen
dc.publisherSoc Study Reproduction
dc.source.urihttps://doi.org/10.1095/biolreprod.103.024828
dc.subjectOvarian Follicle
dc.subjectOocytes
dc.subjectCells, Cultured
dc.subjectBlastocyst
dc.subjectAnimals
dc.subjectCattle
dc.subjectMilrinone
dc.subjectRolipram
dc.subjectFollicle Stimulating Hormone
dc.subjectPhosphoric Diester Hydrolases
dc.subjectIsoenzymes
dc.subjectEnzyme Inhibitors
dc.subjectCell Culture Techniques
dc.subjectMeiosis
dc.subjectEmbryonic Development
dc.subjectFemale
dc.titleEffect of specific phosphodiesterase isoenzyne inhibitors during in vitro maturation of bovine oocytes on meiotic and developmental capacity
dc.typeJournal article
pubs.publication-statusPublished

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