Resolving low-expression cell surface antigens by time-gated orthogonal scanning automated microscopy

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dc.contributor.authorLu, J.
dc.contributor.authorMartin, J.
dc.contributor.authorLu, Y.
dc.contributor.authorZhao, J.
dc.contributor.authorYuan, J.
dc.contributor.authorOstrowski, M.
dc.contributor.authorPaulsen, I.
dc.contributor.authorPiper, J.
dc.contributor.authorJin, D.
dc.date.issued2012
dc.description.abstractWe report a highly sensitive method for rapid identification and quantification of rare-event cells carrying low-abundance surface biomarkers. The method applies lanthanide bioprobes and time-gated detection to effectively eliminate both nontarget organisms and background noise and utilizes the europium containing nanoparticles to further amplify the signal strength by a factor of similar to 20. Of interest is that these nanoparticles did not correspondingly enhance the intensity of nonspecific binding. Thus, the dramatically improved signal-to-background ratio enables the low-expression surface antigens on single cells to be quantified. Furthermore, we applied an orthogonal scanning automated microscopy (OSAM) technique to rapidly process a large population of target-only cells on microscopy slides, leading to quantitative statistical data with high certainty. Thus, the techniques together resolved nearly all false-negative events from the interfering crowd including many false-positive events.
dc.description.statementofresponsibilityJie Lu, Jody Martin, Yiqing Lu, Jiangbo Zhao, Jingli Yuan, Martin Ostrowski, Ian Paulsen, James A. Piper, and Dayong Jin
dc.identifier.citationAnalytical Chemistry, 2012; 84(22):9674-9678
dc.identifier.doi10.1021/Ac302550u
dc.identifier.issn0003-2700
dc.identifier.issn1086-4377
dc.identifier.orcidZhao, J. [0000-0002-5883-6017]
dc.identifier.urihttp://hdl.handle.net/2440/91932
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.granthttp://purl.org/au-research/grants/arc/DP1095465
dc.rights© 2012 American Chemical Society
dc.source.urihttps://doi.org/10.1021/ac302550u
dc.subjectovarian cancer
dc.titleResolving low-expression cell surface antigens by time-gated orthogonal scanning automated microscopy
dc.typeJournal article
pubs.publication-statusPublished

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