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Fast ion chromatography using short anion exchange columns

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dc.contributor.authorTyrrell, É.
dc.contributor.authorShellie, R.A.
dc.contributor.authorHilder, E.F.
dc.contributor.authorPohl, C.A.
dc.contributor.authorHaddad, P.R.
dc.date.issued2009
dc.description.abstractA fast ion chromatographic system is described which uses shorter column lengths and compares various eluent profiles in order to maximise the performance without sacrificing the chromatographic resolution. Both isocratic and gradient elution profiles were considered to find the most efficient mode of separation. The separation and determination of seven target anions (chloride, chlorate, nitrate, chromate, sulfate, thiocyanate and perchlorate) was achieved using a short (4 mm ID, 50 mm long) column packed with Dionex AS20 high-capacity anion exchange material. A hydroxide eluent was used at an initial concentration of 25 mM (at a flow-rate of 1.0 mL/min) and two performance maxima were found. The maximum efficiency occurred at a normalised gradient ramp rate of 5 mM/t0, resulting in a peak capacity of 16, while the fastest separation (<3 min) occurred at a normalised ramp rate of 30 mM/t0. The retention time, peak width and resolution using the different eluent profiles on varying column lengths is also compared. Further investigations in this study determined that the highest peak capacity separation under gradient conditions could be approximated using an isocratic separation. The advantage of using this novel approach to approximate the maximum efficiency separation removes the need for column re-equilibration that is required for gradient elution resulting in faster analyses and enhanced sample throughput, with benefits in particular for multidimensional chromatography.
dc.identifier.citationJournal of Chromatography A, 2009; 1216(48):8512-8517
dc.identifier.doi10.1016/j.chroma.2009.10.003
dc.identifier.issn0021-9673
dc.identifier.issn1873-3778
dc.identifier.urihttps://hdl.handle.net/11541.2/111902
dc.language.isoen
dc.publisherElsevier
dc.relation.fundingARC DP0663781
dc.relation.fundingARC FF0668673
dc.rightsCopyright 2009 Elsevier
dc.source.urihttps://doi.org/10.1016/j.chroma.2009.10.003
dc.subjection chromatography
dc.subjectinorganic anions
dc.subjectfast separation
dc.subjectshort columns
dc.subjectgradient elution
dc.subjectpeak capacity
dc.titleFast ion chromatography using short anion exchange columns
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915981012001831

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