A novel promoter regulates calcitonin receptor gene expression in human osteoclasts

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dc.contributor.authorShen, Z.
dc.contributor.authorCrotti, T.
dc.contributor.authorFlannery, M.
dc.contributor.authorMatsuzaki, K.
dc.contributor.authorGoldring, S.
dc.contributor.authorMcHugh, K.
dc.date.issued2007
dc.description.abstractThe calcitonin receptor (CTR) is expressed in a wide variety of tissues and cell types. In bone, its expression is restricted to osteoclasts, the cells that mediate bone resorption. The human CTR (hCTR) gene has a complex structural organization that exhibits similarity to the porcine (pCTR) and mouse (mCTR) CTR genes. In these species, alternative splicing of a single gene generates multiple CTR isoforms that are distributed in both tissue-specific and species-specific patterns. However, the structural organization of the 5' putative regulatory region and transcriptional mechanisms responsible for tissue-specific expression of the different CTR isoforms are not fully defined. The present studies were undertaken to characterize the structural organization of the 5'-region of the hCTR and identify the regulatory regions involved in osteoclast-specific transcriptional activation. Analysis of mRNA prepared from human osteoclasts using reverse transcription-polymerase chain reaction (RT-PCR) and transient transfection of hCTR promoter-luciferase reporter constructs identified two regions in the 5'-flanking sequence of the hCTR gene that regulated CTR gene expression in osteoclasts. Both of these putative promoters were responsive to the osteoclast-inducing cytokine, receptor activator of NF-kappaB ligand (RANKL) and demonstrated trans-activation by the RANKL-induced transcription factor nuclear factor of activated T cells (NFATc1), consistent with a role in regulating CTR gene expression in osteoclasts.
dc.identifier.citationBiochimica et Biophysica Acta: international journal of biochemistry and biophysics, 2007; 1769(11-12):659-667
dc.identifier.doi10.1016/j.bbaexp.2007.08.005
dc.identifier.issn0006-3002
dc.identifier.issn1878-2434
dc.identifier.orcidCrotti, T. [0000-0002-5422-3758]
dc.identifier.urihttp://hdl.handle.net/2440/48110
dc.language.isoen
dc.publisherElsevier Science
dc.source.urihttps://doi.org/10.1016/j.bbaexp.2007.08.005
dc.subjectCells, Cultured
dc.subjectOsteoclasts
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectReceptors, Calcitonin
dc.subjectRNA, Messenger
dc.subjectTransfection
dc.subjectGene Expression Regulation
dc.subjectTissue Distribution
dc.subjectRANK Ligand
dc.subjectPromoter Regions, Genetic
dc.titleA novel promoter regulates calcitonin receptor gene expression in human osteoclasts
dc.typeJournal article
pubs.publication-statusPublished

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