8 prenylnaringenin novel phytoestrogen, inhibits angiogenesis in vitro and vivo.

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dc.contributor.authorPepper, M.
dc.contributor.authorHazel, S.
dc.contributor.authorHumpel, M.
dc.contributor.authorSchleuning, W.
dc.date.issued2004
dc.description.abstract8-Prenylnaringenin is a recently discovered phytoestrogen. Using an in vitro model of angiogenesis in which endothelial cells can be induced to invade a three-dimensional collagen gel within which they form capillary-like tubes, we demonstrate that 8-prenylnaringenin inhibits angiogenesis induced by basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), or the synergistic effect of the two cytokines in combination, with an IC(50) of between 3 and 10 microM. This effect was seen with bovine microvascular endothelial cells derived from the adrenal cortex (BME cells) and with endothelial cells from the bovine thoracic aorta (BAE cells). The inhibitory effects of 8-prenylnaringenin were found to be roughly equipotent to those of genistein that has previously been shown to inhibit angiogenesis in vitro. Early chorioallantoic membrane (CAM) assay results showed reductions in both vessel lengths and vein diameters, with similar potency in the 8-prenylnaringenin and genistein groups. Similar effects on the CAM vessels were seen when the two substances were co-added. These findings suggest that 8-prenylnaringenin has potential therapeutic applications for diseases in which angiogenesis is an important component.
dc.description.statementofresponsibilityMichael S. Pepper, Susan J. Hazel, Michael Hümpel, Wolf-Dieter Schleuning
dc.identifier.citationJournal of Cellular Physiology, 2004; 199(1):98-107
dc.identifier.doi10.1002/jcp.10460
dc.identifier.issn0021-9541
dc.identifier.issn1097-4652
dc.identifier.orcidHazel, S. [0000-0002-1804-690X]
dc.identifier.urihttp://hdl.handle.net/2440/34624
dc.language.isoen
dc.publisherWiley-Liss
dc.source.urihttps://doi.org/10.1002/jcp.10460
dc.subjectAllantois
dc.subjectChorion
dc.subjectCells, Cultured
dc.subjectEndothelial Cells
dc.subjectAnimals
dc.subjectHumans
dc.subjectFlavanones
dc.subjectIsoflavones
dc.subjectGenistein
dc.subjectGrowth Inhibitors
dc.subjectFibroblast Growth Factor 2
dc.subjectVascular Endothelial Growth Factor A
dc.subjectRecombinant Proteins
dc.subjectPlant Preparations
dc.subjectPhytoestrogens
dc.subjectNeovascularization, Physiologic
dc.subjectIn Vitro Techniques
dc.title8 prenylnaringenin novel phytoestrogen, inhibits angiogenesis in vitro and vivo.
dc.typeJournal article
pubs.publication-statusPublished

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