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Feedback regulation through myosin II confers robustness on RhoA signalling at E-cadherin junctions

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dc.contributor.authorPriya, R.
dc.contributor.authorGomez, G.A.
dc.contributor.authorBudnar, S.
dc.contributor.authorVerma, S.
dc.contributor.authorCox, H.L.
dc.contributor.authorHamilton, N.A.
dc.contributor.authorYap, A.S.
dc.date.issued2015
dc.descriptionData source: Supplementary information, http://www.nature.com/ncb/journal/v17/n10/full/ncb3239.html
dc.description.abstractActomyosin at the epithelial zonula adherens (ZA) generates junctional tension for tissue integrity and morphogenesis. This requires the RhoA GTPase, which establishes a strikingly stable active zone at the ZA. Mechanisms must then exist to confer robustness on junctional RhoA signalling at the population level. We now identify a feedback network that generates a stable mesoscopic RhoA zone out of dynamic elements. The key is scaffolding of ROCK1 to the ZA by myosin II. ROCK1 protects junctional RhoA by phosphorylating Rnd3 to prevent the cortical recruitment of the Rho suppressor, p190B RhoGAP. Combining predictive modelling and experimentation, we show that this network constitutes a bistable dynamical system that is realized at the population level of the ZA. Thus, stability of the RhoA zone is an emergent consequence of the network of interactions that allow myosin II to feedback to RhoA.
dc.identifier.citationNature Cell Biology, 2015; 17(10):1282-1293
dc.identifier.doi10.1038/ncb3239
dc.identifier.issn1465-7392
dc.identifier.issn1476-4679
dc.identifier.urihttps://hdl.handle.net/11541.2/125867
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.fundingNHMRC 1044041
dc.relation.fundingNHMRC 1037320
dc.relation.fundingNHMRC 1067405
dc.relation.fundingOncology Research Foundation The Kids Cancer Project
dc.relation.fundingNational Breast Cancer Foundation (Australia) EMPathy National Collaborative Research Program (CG-10-04)
dc.relation.fundingUniversity of Queensland 2012003354
dc.relation.fundingANZ Trustees PhD Scholarship in Medical Research
dc.rightsCopyright 2015 Macmillan Publishers
dc.source.urihttps://doi.org/10.1038/ncb3239
dc.subjectadherens junctions
dc.subjectcadherins
dc.subjectcell adhesion
dc.subjectRHO signalling
dc.titleFeedback regulation through myosin II confers robustness on RhoA signalling at E-cadherin junctions
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9916123200001831

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