Disengaging spinal afferent nerve communication with the brain in live mice

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dc.contributor.authorKyloh, M.A.
dc.contributor.authorHibberd, T.J.
dc.contributor.authorCastro, J.
dc.contributor.authorHarrington, A.M.
dc.contributor.authorTravis, L.
dc.contributor.authorDodds, K.N.
dc.contributor.authorWiklendt, L.
dc.contributor.authorBrierley, S.M.
dc.contributor.authorZagorodnyuk, V.P.
dc.contributor.authorSpencer, N.J.
dc.date.issued2022
dc.description.abstractOur understanding of how abdominal organs (like the gut) communicate with the brain, via sensory nerves, has been limited by a lack of techniques to selectively activate or inhibit populations of spinal primary afferent neurons within dorsal root ganglia (DRG), of live animals. We report a survival surgery technique in mice, where select DRG are surgically removed (unilaterally or bilaterally), without interfering with other sensory or motor nerves. Using this approach, pain responses evoked by rectal distension were abolished by bilateral lumbosacral L5-S1 DRG removal, but not thoracolumbar T13-L1 DRG removal. However, animals lacking T13-L1 or L5-S1 DRG both showed reduced pain sensitivity to distal colonic distension. Removal of DRG led to selective loss of peripheral CGRP-expressing spinal afferent axons innervating visceral organs, arising from discrete spinal segments. This method thus allows spinal segment-specific determination of sensory pathway functions in conscious, free-to-move animals, without genetic modification.
dc.description.statementofresponsibilityMelinda A. Kyloh, Timothy J. Hibberd, Joel Castro, Andrea M. Harrington, Lee Travis, Kelsi N. Dodds, Lukasz Wiklendt, Stuart M. Brierley, Vladimir P. Zagorodnyuk and Nick J. Spencer
dc.identifier.citationCommunications Biology, 2022; 5(1):915-1-915-15
dc.identifier.doi10.1038/s42003-022-03876-x
dc.identifier.issn2399-3642
dc.identifier.issn2399-3642
dc.identifier.orcidCastro, J. [0000-0002-5781-2224]
dc.identifier.orcidHarrington, A.M. [0000-0002-1562-4137]
dc.identifier.orcidDodds, K.N. [0000-0003-0555-868X]
dc.identifier.orcidBrierley, S.M. [0000-0002-2527-2905]
dc.identifier.urihttps://hdl.handle.net/2440/136558
dc.language.isoen
dc.publisherSpringer
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1156427
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1184546
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/2008727
dc.relation.granthttp://purl.org/au-research/grants/arc/DP180101395
dc.relation.granthttp://purl.org/au-research/grants/arc/DP220100070
dc.rights© The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.
dc.source.urihttps://doi.org/10.1038/s42003-022-03876-x
dc.subjectPain
dc.subject.meshColon
dc.subject.meshBrain
dc.subject.meshGanglia, Spinal
dc.subject.meshAnimals
dc.subject.meshMice
dc.subject.meshPain
dc.titleDisengaging spinal afferent nerve communication with the brain in live mice
dc.typeJournal article
pubs.publication-statusPublished

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