Expression of Plasmodium falciparum blood-stage antigens in Escherichia coli: detection with antibodies from immune humans

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dc.contributor.authorKemp, D.J.
dc.contributor.authorCoppel, R.L.
dc.contributor.authorCowman, A.F.
dc.contributor.authorSaint, R.B.
dc.contributor.authorBrown, G.V.
dc.contributor.authorAnders, R.F.
dc.date.issued1983
dc.description.abstractMany proteins produced by blood stages of the malaria parasite Plasmodium falciparum are natural immunogens in man. As an approach to determining which of these are relevant to protective immunity we have constructed an expression library of P. falciparum cDNA sequences, cloned in Escherichia coli. The cDNA sequences were inserted into the beta-galactosidase gene of an ampicillin-resistant derivative of the temperature-sensitive lysogenic bacteriophage lambda gt11. About 5% of the resulting clones expressed P. falciparum sequences as polypeptides fused to beta-galactosidase. We have identified many clones that express P. falciparum antigens by immunological screening in situ with antibodies from immune human sera that inhibit P. falciparum growth in vitro. The antigen-positive clones contain P. falciparum cDNA sequences, as determined by hybridization. Some express polypeptides that are larger than beta-galactosidase and react both with antibodies to beta-galactosidase and with antibodies from humans immune to P. falciparum. The cloned P. falciparum antigens should facilitate new approaches to the identification of potential vaccine molecules.
dc.description.statementofresponsibilityDavid J. Kemp, Ross L. Coppel, Alan F. Cowman, Robert B. Saint, Graham V. Brown, and Robin F. Anders
dc.identifier.citationProceedings of the National Academy of Sciences of the United States of America, 1983; 80(12):3787-3791
dc.identifier.doi10.1073/pnas.80.12.3787
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.urihttp://hdl.handle.net/2440/85671
dc.language.isoen
dc.publisherNational Academy of Sciences
dc.rightsCopyright status unknown
dc.source.urihttps://doi.org/10.1073/pnas.80.12.3787
dc.subjectAnimals
dc.subjectHumans
dc.subjectPlasmodium falciparum
dc.subjectEscherichia coli
dc.subjectMalaria
dc.subjectDNA Restriction Enzymes
dc.subjectbeta-Galactosidase
dc.subjectDNA
dc.subjectRNA, Messenger
dc.subjectImmune Sera
dc.subjectAntigens
dc.subjectImmunity
dc.subjectBase Sequence
dc.subjectPlasmids
dc.titleExpression of Plasmodium falciparum blood-stage antigens in Escherichia coli: detection with antibodies from immune humans
dc.typeJournal article
pubs.publication-statusPublished

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