Deletion of creB in Aspergillus oryzae increases secreted hydrolytic enzyme activity

Simple item page
dc.contributor.authorHunter, A.
dc.contributor.authorMorris, T.
dc.contributor.authorJin, B.
dc.contributor.authorSaint, C.
dc.contributor.authorKelly, J.
dc.date.issued2013
dc.description.abstractAspergillus oryzae has been used in the food and beverage industry for centuries, and industrial strains have been produced by multiple rounds of selection. Targeted gene deletion technology is particularly useful for strain improvement in such strains, particularly when they do not have a well-characterized meiotic cycle. Phenotypes of an Aspergillus nidulans strain null for the CreB deubiquitinating enzyme include effects on growth and repression, including increased activity levels of various enzymes. We show that Aspergillus oryzae contains a functional homologue of the CreB deubiquitinating enzyme and that a null strain shows increased activity levels of industrially important secreted enzymes, including cellulases, xylanases, amylases, and proteases, as well as alleviated inhibition of spore germination on glucose medium. Reverse transcription-quantitative PCR (RT-qPCR) analysis showed that the increased levels of enzyme activity in both Aspergillus nidulans and Aspergillus oryzae are mirrored at the transcript level, indicating transcriptional regulation. We report that Aspergillus oryzae DAR3699, originally isolated from soy fermentation, has a similar phenotype to that of a creB deletion mutant of the RIB40 strain, and it contains a mutation in the creB gene. Collectively, the results for Aspergillus oryzae, Aspergillus nidulans, Trichoderma reesei, and Penicillium decumbens show that deletion of creB may be broadly useful in diverse fungi for increasing production of a variety of enzymes.
dc.description.statementofresponsibilityA. J. Hunter, T. A. Morris, B. Jin, C. P. Saint, J. M. Kelly
dc.identifier.citationApplied and Environmental Microbiology, 2013; 79(18):5480-5487
dc.identifier.doi10.1128/AEM.01406-13
dc.identifier.issn0099-2240
dc.identifier.issn1098-5336
dc.identifier.urihttp://hdl.handle.net/2440/80340
dc.language.isoen
dc.publisherAmer Soc Microbiology
dc.relation.granthttp://purl.org/au-research/grants/arc/LP0562153
dc.relation.granthttp://purl.org/au-research/grants/arc/LP0562153
dc.rightsCopyright © 2013, American Society for Microbiology. All Rights Reserved.
dc.source.urihttps://doi.org/10.1128/aem.01406-13
dc.subjectSpores, Fungal
dc.subjectAspergillus nidulans
dc.subjectAspergillus oryzae
dc.subjectPenicillium
dc.subjectTrichoderma
dc.subjectHydro-Lyases
dc.subjectGene Expression Profiling
dc.subjectGene Deletion
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectUbiquitin-Specific Proteases
dc.titleDeletion of creB in Aspergillus oryzae increases secreted hydrolytic enzyme activity
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Molecular and Biomedical Science publications