Mitogenic effects of growth hormone in cultured human fibroblasts: evidence for action via local insulin-like growth factor 1 production

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dc.contributor.authorCouper, J.
dc.contributor.authorHaynes, K.
dc.contributor.authorWerther, G.
dc.date.issued1988
dc.description.abstractWe examined human growth hormone's (hGH) effect on mitogenesis in cbltured human fibroblasts, and the role of local insulin-like growth factor I (IGF-I). With 0.5% human hypopituitary serum (HPS), hGH increased thymidine incorporation (TI) over serum-free medium dose responsively, with half-maximal effect at 10 ng/ml (0.5 nM) (hGH 127+/-8.8%; IGF-I 107+/-(1.7% [SEMI) (n = 10). Similarly, with 0.5% HPS, hGH and IGF-I increased cell replication by 172+/-8.2% and 169+/-25%, respectively (n = 4). Specific IGF-I monoclonal antibody (Sml.2) dose dependently blunted TI stimulated by 10 ng/ml hGH or IGF-I (at 1:1000, 38+/-6.5% and 30+/-14% reduction, respectively). Sml.2 also reduced cell replication by both 10 ng/ml hGH and IGF-I, respectively, to 32% and 42% of stimulated values. Dexamethasone (0.1 MiM) synergistically enhanced TI by both IGF-I and hGH. A 28-h time course for TI showed that hGH stimulated a similar peak to IGF-I, lagging in its effect by 4-10 h. We have provided further evidence that hGH stimulates growth of cultured human fibroblasts via local IGF-I production, consistent with IGF-I's paracrine-autocrine role.
dc.description.statementofresponsibilityJ. J. Cook, K. M. Haynes, and G. A. Werther
dc.identifier.citationJournal of Clinical Investigation, 1988; 81(1):206-212
dc.identifier.doi10.1172/JCI113296
dc.identifier.issn0021-9738
dc.identifier.orcidCouper, J. [0000-0003-4448-8629]
dc.identifier.urihttp://hdl.handle.net/2440/116178
dc.language.isoen
dc.publisherAmerican Society for Clinical Investigation
dc.rights© The American Society for Clinical Investigation
dc.subjectCells, Cultured
dc.subjectFibroblasts
dc.subjectHumans
dc.subjectHypopituitarism
dc.subjectGrowth Hormone
dc.subjectSomatomedins
dc.subjectInsulin-Like Growth Factor I
dc.subjectThymidine
dc.subjectMitogens
dc.subjectCell Division
dc.subjectAdult
dc.subjectMale
dc.titleMitogenic effects of growth hormone in cultured human fibroblasts: evidence for action via local insulin-like growth factor 1 production
dc.typeJournal article
pubs.publication-statusPublished

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