Effects of estradiol and dihydrotestosterone on osteoblast gene expression in osteopenic ovariectomized rats

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dc.contributor.authorDavey, R.
dc.contributor.authorMorris, H.
dc.date.issued2005
dc.description.abstractBecause androgens increase bone formation and estrogens inhibit bone resorption, there is a potential therapeutic use for a combined treatment of these hormones to preserve bone. We investigated the effect of dihydrotestosterone (DHT) and estradiol, alone and in combination, on the mRNA levels of genes expressed during osteoblast development in osteopenic ovariectomized (ovx) rats: 40 animals were ovx and administered vehicle or 80 mg/kg body weight DHT at 15 weeks postovariectomy. At 19 weeks postovariectomy, the rats were administered vehicle or 20 mg/kg body weight estradiol for 1 week. The treatment groups were as follows: (1) vehicle + vehicle, (2) DHT + vehicle, (3) vehicle + estradiol, and (4) DHT + estradiol. Fasting blood and urine samples were collected at 15, 17, 19, and 20 weeks postovariectomy for bone biochemical analyses. On completion of both procedures, the long bones were removed and total RNA extracted. Combined DHT and estradiol treatment increased the mRNA (P < 0.001) and serum levels of alkaline phosphatase (ALP) (P < 0.01) compared to control rats. These data suggest that combined DHT and estradiol treatment stimulates osteoblasts at an early stage of their development when ALP is expressed.
dc.identifier.citationJournal of Bone and Mineral Metabolism, 2005; 23(3):212-218
dc.identifier.doi10.1007/s00774-004-0586-z
dc.identifier.issn0914-8779
dc.identifier.issn1435-5604
dc.identifier.orcidMorris, H. [0000-0002-2745-3750]
dc.identifier.urihttp://hdl.handle.net/2440/27496
dc.language.isoen
dc.publisherSpringer-Verlag Tokyo
dc.source.urihttps://doi.org/10.1007/s00774-004-0586-z
dc.subjectOsteoblasts
dc.subjectAnimals
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectBone Diseases, Metabolic
dc.subjectDisease Models, Animal
dc.subjectCalcium
dc.subjectCreatine
dc.subjectDihydrotestosterone
dc.subjectEstradiol
dc.subjectSerum Albumin
dc.subjectRNA, Messenger
dc.subjectDrug Therapy, Combination
dc.subjectOvariectomy
dc.subjectGene Expression Regulation
dc.subjectFemale
dc.titleEffects of estradiol and dihydrotestosterone on osteoblast gene expression in osteopenic ovariectomized rats
dc.typeJournal article
pubs.publication-statusPublished

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